Homeobox C8 inhibited the osteo‐/dentinogenic differentiation and migration ability of stem cells of the apical papilla via activating KDM1A
Abstract
Enhancing the functions of mesenchymal stem cells (MSCs) is considered a potential approach for promoting tissue regeneration. In the present study, we investigate the role of HOXC8 in regulating differentiation and migration by using stem cells of the apical papilla (SCAPs). Our results showed that overexpression of HOXC8 suppressed the osteo‐/dentinogenic differentiation, as detected by measuring alkaline phosphatase activity, in vitro mineralization, and the expressions of dentin sialophosphoprotein, dentin matrix acidic phosphoprotein 1, bone sialoprotein, runt‐related transcription factor 2, and osterix in SCAPs, and inhibited in vivo osteo‐/dentinogenesis of SCAPs. In addition, knockdown of HOXC8 promoted the osteo‐/dentinogenic differentiation potentials of SCAPs. Mechanically, HOXC8 enhanced KDM1A transcription by directly binding to its promoter. HOXC8 and KDM1A also inhibited the migration and chemotaxis abilities of SCAPs. To sum up, HOXC8 negatively regulated the osteo‐/dentinogenic differentiation and migration abilities of SCAPs by directly enhancing KDM1A transcription and indicated that HOXC8 and KDM1A could serve as potential targets for enhancing dental MSC mediated tissue regeneration.
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Multiple homeobox genes have been implicated in bone mass and osteogenesis regulation.
“Multiple homeobox genes have been implicated in the regulation of bone mass and osteogenesis16–19.”
From Targeting Msx2 as a brake in the fusion fate of osteoclasts and an anabolic therapy in pre-clinical models of osteoporosis (2025) · cites this paper as [17]
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References 52 · Cited by 30
- Authors
- Yang, H., Liang, Y., Cao, Y., Cao, Y., & Fan, Z.
- Affiliations
- Capital Medical University (CN)
- Corresponding author
- Cao, Yu
- Fan, Zhipeng
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 2020-04-04
- Volume
- 235
- Issue
- 11
- Pages
- 8432-8445
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W3014873165
- Cited by
- 30
- References
- 52
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 3 · 2023: 9 · 2022: 5 · 2021: 3 · 2020: 3
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cellular Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81670948, 81625005)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- HOXC8, LSD1, osteogenic differentiation, dentinogenic differentiation, cell migration, chemotaxis, transcriptional regulation, dental mesenchymal stem cells, tooth regeneration, demethylation
- MeSH terms
- Cell Differentiation, Cells, Cultured, Dental Papilla, Genes, Homeobox, Humans, Osteogenesis, Stem Cells, Homeodomain Proteins, Cell Proliferation, Histone Demethylases, Mesenchymal Stem Cells
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