Phenotypic differences between people varying in muscularity

Abstract

Background: Body mass is the primary metabolic compartment related to a vast number of clinical indices and predictions. The extent to which skeletal muscle (SM), a major body mass component, varies between people of the same sex, weight, height, and age is largely unknown. The current study aimed to explore the magnitude of muscularity variation present in adults and to examine if variation in muscularity associates with other body composition and metabolic measures. Methods: Muscularity was defined as the difference (residual) between a person's actual and model‐predicted SM mass after controlling for their weight, height, and age. SM prediction models were developed using data from a convenience sample of 492 healthy non‐Hispanic (NH) White adults (ages 18–80 years) who had total body SM and SM surrogate, appendicular lean soft tissue (ALST), measured with magnetic resonance imaging and dual‐energy X‐ray absorptiometry, respectively; residual SM (SM R ) and ALST were expressed in kilograms and kilograms per square meter. ALST mass was also evaluated in a population sample of 8623 NH‐White adults in the 1999–2006 National Health and Nutrition Examination Survey. Associations between muscularity and variation in the residual mass of other major organs and tissues and resting energy expenditure were evaluated in the convenience sample. Results: The SM, on average, constituted the largest fraction of body weight in men and women up to respective BMIs of 35 and 25 kg/m 2 . SM in the convenience sample varied widely with a median of 31.2 kg and an SM R inter‐quartile range/min/max of 3.35 kg/−10.1 kg/9.0 kg in men and 21.1 kg and 2.59 kg/−7.2 kg/7.5 kg in women; per cent of body weight as SM at 25th and 75th percentiles for men were 33.1% and 39.6%; corresponding values in women were 24.2% and 30.8%; results were similar for SM R indices and for ALST measures in the convenience and population samples. Greater muscularity in the convenience sample was accompanied by a smaller waist circumference (men/women: P < 0.001/=0.085) and visceral adipose tissue ( P = 0.014/0.599), larger liver ( P = 0.065/<0.001), kidneys ( P = 0.051/<0.009), and bone mineral ( P < 0.001/<0.001), and larger magnitude resting energy expenditure ( P < 0.001/<0.001) than predicted for the same sex, age, weight, and height. Conclusions: Muscle mass is the largest body compartment in most adults without obesity and is widely variable in mass across people of similar body size and age; and high muscularity is accompanied by distinct body composition and metabolic characteristics. This previously unrecognized heterogeneity in muscularity in the general population has important clinical and research implications.