Fate of donor bone marrow cells in medial collateral ligament after simulated autologous transplantation
Open accessAbstract
A potential strategy to enhance ligament healing by transplantation of mesenchymal stem cells (MSCs), which are demonstrated to differentiate into fibroblast‐like cells in vitro, is presented. The objective of this study was to follow transplanted nucleated cells from bone marrow, which contain MSCs, in the healing medial collateral ligament (MCL) over time, and to examine their phenotype and survivability. It was hypothesized that MSCs in nucleated cells from bone marrow would differentiate into fibroblast‐like cells in the healing ligament following adaptation to the environment. The transplantation model employed in this study eliminates the immune response to a donor by the recipient using a transgenic rat (donor), which does not produce foreign protein from transgenes, and its wild‐type rat (recipient) in order to simulate autologous transplantation. The MCL of the wild‐type rat was ruptured, where 1 × 10 6 nucleated cells of bone marrow from the transgenic rat were injected. The transgenes in transplanted nucleated cells were detected throughout the healing MCL for 28 days by in situ hybridization. At 3 days, many donor cells were evident in the injury site and fascial pocket, and some were found in the midsubstance. Morphologically, transplanted cells with elongated nuclei were found at the ruptured edge of the midsubstance and surface of the unruptured site after 3 days. At 28 days, these cells continued to survive in the healing MCL. Their shapes were similar to those of surrounding recipient MCL fibroblasts. Thus, transplanted cells might differentiate into fibroblasts. Therefore, it was demonstrated that there is a potential for nucleated cells from bone marrow to serve as a vehicle for therapeutic molecules as well as to be a source in enhancing healing of ligaments. Microsc. Res. Tech. 58:39–44, 2002. © 2002 Wiley‐Liss, Inc.
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There is potential for MSCs from bone marrow to serve as therapeutic molecules and enhance MCL healing.
“The authors demonstrated that there is the potential for MSCs from the bone marrow to serve as therapeutic molecules, as well as to enhance MCL healing.”
From Mesenchymal Stem Cells for Enhanced Healing of the Medial Collateral Ligament of the Knee Joint (2023) · cites this paper as [60]
The Discussion (page not supplied) says bone-marrow cells containing MSCs could serve as a molecular vehicle and a source for healing ligaments, supporting their potential role in MCL healing. It does not say MSCs themselves serve as therapeutic molecules; it describes them as a vehicle for such molecules. (LLM, conf=0.87)
Experimental — accuracy not yet measuredMSCs differentiated into fibroblast-like cells after transplantation into the healing ligament of the recipient and continued to survive and migrate in the surrounding healing MCL until 4 weeks.
“The results revealed that MSCs differentiated into fibroblast-like cells after transplantation into the healing ligament of the recipient. These cells were similar to those of the surrounding recipient MCL fibroblasts in morphology and continued to survive and migrate in the surrounding healing MCL until 4 weeks.”
From Mesenchymal Stem Cells for Enhanced Healing of the Medial Collateral Ligament of the Knee Joint (2023) · cites this paper as [60]
The Results and Discussion report that transplanted bone-marrow nucleated cells survived in the healing MCL for 28 days, appeared at sites away from the injury, and developed a morphology similar to recipient fibroblasts. The paper says these cells contained MSCs, but did not identify which donor cells were MSCs; it presents migration as a possibility rather than directly demonstrating it. (LLM, conf=0.85)
Experimental — accuracy not yet measuredPreclinical studies regarding MSC-based approaches in MCL injuries have shown promising results.
“Some preclinical studies regarding MSC-based approaches in MCL injuries have shown promising results.”
From Mesenchymal Stem Cells for Enhanced Healing of the Medial Collateral Ligament of the Knee Joint (2023) · cites this paper as [60]
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References 39 · Cited by 69
- Authors
- Watanabe, N., Woo, S. L., Papageorgiou, C., Celechovsky, C., & Takai, S.
- Affiliations
- Kyoto Prefectural University of Medicine (JP)
- University of Pittsburgh (US)
- Corresponding author
- Woo, Savio L‐Y.
- Venue
- Microscopy Research and Technique
- Publisher
- Wiley
- Published
- 2002-06-21
- Volume
- 58
- Issue
- 1
- Pages
- 39-44
- ISSN
- 1059-910X (ISSN-L)
- 1097-0029
- OpenAlex
- W2151265869
- Cited by
- 69
- References
- 39
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 3 · 2022: 1 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 2 · 2015: 4 · 2013: 2 · 2012: 2
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Microscopy Research and Technique (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR41820, R01AR041820)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Keywords
- mesenchymal stem cells, autologous transplantation, bone marrow nucleated cells, medial collateral ligament, ligament healing, transgenic rats, transgene detection, cell survival, fibroblast differentiation, cell tracking, ligament rupture, cell therapy
- MeSH terms
- Animals, Bone Marrow Cells, Cell Count, Female, Fibroblasts, Genetic Vectors, Mesoderm, Transplantation, Autologous, Wound Healing, Genetic Therapy, Bone Marrow Transplantation, Medial Collateral Ligament, Knee, Models, Animal, Animals, Genetically Modified, Rats
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