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Cited by 7Advanced Neuroimaging Techniques and ApplicationsGlaucoma and retinal disordersMultiple Sclerosis Research Studies

Anterograde-propagation of axonal degeneration in the visual system of wlds mice characterized by diffusion tensor imaging: DTI and Gd-T1WI of RGC Axonal Degeneration

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Sun, Shu-Wei, Nishioka, Christopher, Chung, Chen-Fang, Park, JoAnn, Liang, Hsiao-Fang·2017·Journal of Magnetic Resonance Imaging
DOI 10.1002/jmri.25368PMC PMC6426298
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Abstract

Purpose To evaluate the feasibility of using diffusion tensor imaging (DTI) to characterize the temporospatial profile of axonal degeneration and its relation to blood–brain barrier (BBB) permeability. Materials and Methods Longitudinal DTI was performed in Wallerian degeneration slow (WldS) mice following retinal ischemia. In parallel, gadolinium (Gd)-enhanced T1-weighted imaging (Gd-T1WI) was performed to evaluate BBB permeability in white matter during axonal degeneration. To confirm the in vivo findings, immunohistochemistry using SMI-31 and myelin basic protein (MBP) was performed to examine the axons and myelin, respectively, and Evans blue was used to evaluate the permeability of the BBB. Results Reduced axial diffusivity was found in the optic nerve (ON, –15%, P = 0.0063) 1 week and optic tact (OT, –18%, P = 0.0077) 2 weeks after retinal ischemia, which were respectively associated with an 11% (P = 0.0116) and 25% (P = 0.0001) axonal loss. Increased radial diffusivity was found 1–2 weeks after the colocated decrease of axial diffusivity (35% increase, P = 0.0388 in the ON at week 2 and an 80% increase, P = 0.0015 in the OT at week 4). No significant changes were observed using Gd-T1WI (P = 0.13–0.75), although an approximately 1-fold increase in Evans blue staining intensity was found in the injured ON and OT starting 1 week after retinal ischemia. Conclusion We demonstrated the utility of DTI to characterize anterograde-propagating axonal degeneration through the ON and OT following retinal damage. Evans blue staining revealed serum albumin accumulation at injured sites, although there was no BBB leakage detectable using Gd-T1WI. Level of Evidence: 2 J. Magn. Reson. Imaging 2017;45:482–491.

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  • Evidence from AD tissue suggests early axonal dysfunction precedes overt cell loss, including dystrophic axons, dysfunctional axonal transport and cytoskeletal abnormalities early in the disease process.

    “This idea is bolstered by evidence from AD tissue, suggestive of early axonal dysfunction preceding overt cell loss. This includes the presence of dystrophic axons, dsyfunctional axonal transport and cytoskeletal abnormalities early in the disease process 62,63.”

    From Amyloid-beta induced retrograde axonal degeneration in a mouse tauopathy model (2019) · cites this paper as [63]

    The Introduction and Discussion describe axonal degeneration after retinal ischemia in mice; the Discussion also mentions cytoskeletal disintegration in a rat model. Neither section reports findings from Alzheimer’s disease tissue or establishes that dystrophic axons, impaired axonal transport, and cytoskeletal abnormalities precede cell loss in that disease. Searches for those features and their timing yielded no supporting passage in our copy. (LLM, conf=0.76)

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Cited by

Sorted by citations

Authors
Sun, S.-W., Nishioka, C., Chung, C.-F., Park, J., & Liang, H.-F.
Affiliations
  • University of California, Riverside (US)
  • Loma Linda University (US)
Corresponding author
Sun, Shu-Wei
Venue
Journal of Magnetic Resonance Imaging
Publisher
Wiley
Published
2016-07-04
Volume
45
Issue
2
Pages
482-491
ISSN
1053-1807 (ISSN-L)
DOI
10.1002/jmri.25368
PMC
PMC6426298
OpenAlex
W2464443837
Cited by
7
References
1
Citations by year
2025: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2
FWCI
0.87
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Magnetic Resonance Imaging (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (R01 NS062830)
  • National Space Biomedical Research Institute (RE03701)
  • through National Aeronautics and Space Administration NCC 9-58
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
  • Health Sciences › Medicine › Ophthalmology › Glaucoma and retinal disorders
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
Keywords
axonal degeneration, retinal ischemia, optic nerve, optic tract, blood-brain barrier permeability, diffusion tensor imaging, axial diffusivity, radial diffusivity, Evans blue staining, myelin basic protein, axonal loss
MeSH terms
Animals, Axons, Blood-Brain Barrier, Feasibility Studies, Female, Retinal Degeneration, Sensitivity and Specificity, Visual Pathways, Wallerian Degeneration, Reproducibility of Results, Diffuse Axonal Injury, Mice, Diffusion Tensor Imaging

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