The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) of membrane proteins and non‐covalent complexes
Abstract
Matrix‐assisted laser desorption/ionization (MALDI) mass spectra and methods to improve their quality are reported for three hydrophobic, membrane‐bound proteins: porin from Escherichia coli, bacteriorhodopsin from Halobacterium salinarium and cholesterolesterase from Pseudomonas fluorescens. Several commonly used UV and IR matrices have been tested. In addition, the susceptibility of MALDI mass spectrometry to various neutral and ionic detergents, known usually to degrade the quality of MALDI mass spectra, has been tested systematically. For porin, consisting of three identical non‐covalently bound subunits, a new sample preparation is reported, resulting in the desorption of the intact quaternary protein structure. This leads to a better understanding of the way a given analyte is embedded into the host matrix crystals.
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Citations
Cites
Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers.
Hillenkamp, F., Karas, M., Beavis, R. C., & Chait, B. T. (1991) · Analytical Chemistry · doi:10.1021/ac00024a716 · Cited by 1,930
Self-citationCovey, T. R., Huang, E. C., & Henion, J. D. (1991) · Analytical Chemistry · doi:10.1021/ac00013a003 · Cited by 446
Cited by
Protein Complexes in the Gas Phase: Technology for Structural Genomics and Proteomics
Benesch, J. L. P., Ruotolo, B. T., Simmons, D. A., & Robinson, C. V. (2007) · Chemical Reviews · doi:10.1021/cr068289b · Cited by 408
Yu, Y.-Q., Gilar, M., Lee, P. J., Bouvier, E. S. P., & Gebler, J. C. (2003) · Analytical Chemistry · doi:10.1021/ac0346196 · Cited by 313
The benefits (and misfortunes) of SDS in top-down proteomics
Kachuk, C., & Doucette, A. A. (2018) · Journal of Proteomics · doi:10.1016/j.jprot.2017.03.002 · Cited by 39
Metadata
- Authors
- Rosinke, B., Strupat, K., Hillenkamp, F., Rosenbusch, J., Dencher, N., Krüger, U., & Galla, H.
- Venue
- Journal of Mass Spectrometry
- Publisher
- Wiley
- Published
- 1995-10
- Volume
- 30
- Issue
- 10
- Pages
- 1462-1468
- ISSN
- 1076-5174 (ISSN-L)
- 1096-9888
- OpenAlex
- W2069746331
- Cited by
- 185
- References
- 2
- FWCI
- 7.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Rosinke, B., Strupat, K., Hillenkamp, F., Rosenbusch, J., Dencher, N., Krüger, U., & Galla, H. (1995). Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) of membrane proteins and non‐covalent complexes. Journal of Mass Spectrometry. https://doi.org/10.1002/jms.1190301012
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
