Asiatic acid, a pentacyclic triterpene from Centella asiatica, is neuroprotective in a mouse model of focal cerebral ischemia
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Asiatic acid, a triterpenoid derivative from Centella asiatica, has shown biological effects such as antioxidant, antiinflammatory, and protection against glutamate- or beta-amyloid-induced neurotoxicity. We investigated the neuroprotective effect of asiatic acid in a mouse model of permanent cerebral ischemia. Various doses of asiatic acid (30, 75, or 165 mg/kg) were administered orally at 1 hr pre- and 3, 10, and 20 hr postischemia, and infarct volume and behavioral deficits were evaluated at day 1 or 7 postischemia. IgG (blood-brain barrier integrity) and cytochrome c (apoptosis) immunostaining was carried out at 24 hr postischemia. The effect of asiatic acid on stress-induced cytochrome c release was examined in isolated mitochondrial fractions. Furthermore, its effects on cell viability and mitochondrial membrane potential were studied in HT-22 cells exposed to oxygen-glucose deprivation. Asiatic acid significantly reduced the infarct volume by 60% at day 1 and by 26% at day 7 postischemia and improved neurological outcome at 24 hr postischemia. Our studies also showed that the neuroprotective properties of asiatic acid might be mediated in part through decreased blood-brain barrier permeability and reduction in mitochondrial injury. The present study suggests that asiatic acid may be useful in the treatment of cerebral ischemia.
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Compounds in CA such as ferulic acid and asiatic acid induce Nrf2 and its ARE-regulated targets, exhibiting a neuroprotective effect.
“Compounds such as the phenolic ferulic acid [89,90] and the triterpene asiatic acid [2,91], induce Nrf2 and its ARE-regulated targets, exhibiting a neuroprotective effect [92,93,94,95].”
From Centella Asiatica Improves Memory and Promotes Antioxidative Signaling in 5XFAD Mice (2019) · cites this paper as [91]
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References 40 · Cited by 155
- Authors
- Krishnamurthy, R. G., Senut, M., Zemke, D., Min, J., Frenkel, M. B., Greenberg, E. J., Yu, S., Ahn, N., Goudreau, J., Kassab, M., Panickar, K. S., & Majid, A.
- Affiliations
- Michigan State University (US)
- United States Department of Agriculture (US)
- Corresponding author
- Majid, Arshad
- Venue
- Journal of Neuroscience Research
- Publisher
- Wiley
- Published
- 2009-04-20
- Volume
- 87
- Issue
- 11
- Pages
- 2541-2550
- ISSN
- 0360-4012 (ISSN-L)
- 1097-4547
- PMC
- PMC2941770
- OpenAlex
- W2079678859
- Cited by
- 155
- References
- 40
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 9 · 2021: 11 · 2020: 9 · 2019: 8 · 2018: 14 · 2017: 14 · 2016: 17 · 2015: 11 · 2014: 8 · 2013: 7 · 2012: 8
- FWCI
- 12.22
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neuroscience Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Aging (T32AG023480, P30AG028717)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Keywords
- asiatic acid, Centella asiatica, pentacyclic triterpenes, neuroprotection, focal cerebral ischemia, ischemic stroke, infarct size, neurological outcome, blood-brain barrier permeability, cytochrome c release, mitochondrial injury, oxygen-glucose deprivation, HT22 cells, mitochondrial membrane potential, apoptosis
- MeSH terms
- Animals, Brain, Cell Line, Cell Survival, Brain Ischemia, Disease Models, Animal, Glucose, Immunoglobulin G, Male, Mice, Inbred C57BL, Mitochondria, Severity of Illness Index, Time Factors, Triterpenes, Cell Hypoxia, Treatment Outcome, Neuroprotective Agents, Infarction, Middle Cerebral Artery, Cytochromes c, Mice, Membrane Potential, Mitochondrial, Pentacyclic Triterpenes
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