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Cited by 50Microbial bioremediation and biosurfactantsSurfactants and Colloidal SystemsEnvironmental Chemistry and Analysis

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Isolation and molecular characterization of biosurfactant producing yeasts from the soil samples contaminated with petroleum derivatives

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Yalçın, Hüsniye T., Ergin‐Tepebaşı, Gülşah, Uyar, Ebru·2018·Journal of Basic Microbiology
DOI 10.1002/jobm.201800126
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Abstract

In this study, 65 yeast strains were isolated from different environmental samples contaminated with various petroleum hydrocarbons such as activated sludges and soil samples from automobile workshops. The yeast isolates were tested for biosurfactant production using various screening methods such as parafilm M test, oil displacement assay, drop collapse assay, determination of surface tension reduction, and emulsification index. Nineteen of the isolates were found positive for biosurfactant production and their molecular characterizations were carried out by sequencing analysis of the ITS1‐5.8S‐ITS2 region and D1/D2 domain of 26S rDNA. The results indicated that these strains were from a wide range of yeast genera including Rhodotorula , Candida , Yarrowia , Geotrichum , Galactomyces , and Cystobasidium . The studies to determine the emulsification index revealed that the biosurfactants produced by Yarrowia lipolytica strains (TEMGS33, TEMOS12, and TEMOS14) and Apiotrichum loubieri strain (TEMOS16) were the most potent and capable of forming stable emulsions with emulsion index (E 24 ) up to 68%. In addition, quantitative measurements of the surface tension reduction of the biosurfactants produced by these strains were carried out by Du Noüy ring method. Biosurfactants produced from Yarrowia lipolytica strain TEMGS33 and Apiotrichum loubieri strain TEMOS16 gave the best results reducing the surface tension to 34.7 ± 1.15 and 35.3 ± 0.55 mN m −1 , respectively. Based on these data, biosurfactants from Yarrawia lipolytica strains (TEMGS33, TEMOS12, and TEMOS14) and Apiotrichum loubieri strain (TEMOS16) showed promising results and might be implemented in numerous industrial fields such as bioremediation and food industry.

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Metadata

Authors
Yalçın, H. T., Ergin‐Tepebaşı, G., & Uyar, E.
Affiliations
  • Ege University (TR)
  • Harran University (TR)
Corresponding author
  • Yalçın, Hüsniye T.
  • Uyar, Ebru
ORCID
Venue
Journal of Basic Microbiology
Publisher
Wiley
Published
2018-06-25
Volume
58
Issue
9
Pages
782-792
ISSN
  • 0233-111X (ISSN-L)
  • 1521-4028
DOI
10.1002/jobm.201800126
OpenAlex
W2810079740
Cited by
50
References
0
Citations by year
2026: 6 · 2025: 5 · 2024: 6 · 2023: 9 · 2022: 10 · 2021: 10 · 2020: 1 · 2019: 2 · 2018: 1
FWCI
1.31
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Basic Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Scientific Research Unit of Ege University (2015-FEN-014)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Surfactants and Colloidal Systems
  • Physical Sciences › Environmental Science › Environmental Chemistry › Environmental Chemistry and Analysis
Keywords
biosurfactants, yeast isolates, oil-contaminated soil, Yarrowia lipolytica, emulsification index, surface tension reduction, emulsion stability, bioremediation, activated sludge, Rhodotorula, Candida, Geotrichum
MeSH terms
Biodegradation, Environmental, DNA, Fungal, DNA, Ribosomal, Emulsions, Hydrocarbons, Petroleum, Phylogeny, Sewage, Soil, Soil Microbiology, Soil Pollutants, Surface Tension, Surface-Active Agents, Turkey, Yeasts, Sequence Analysis, DNA

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