The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Temporal growth factor release from platelet-rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression
Open accessAbstract
Platelet‐rich plasma (PRP) has generated substantial interest for tendon and ligament regeneration because of the high concentrations of growth factors in platelet α‐granules. This study compared the temporal release of growth factors from bone marrow aspirate (BMA), PRP, and lyophilized platelet product (PP), and measured their effects on tendon and ligament gene expression. Blood and BMA were collected and processed to yield PRP and plasma. Flexor digitorum superficialis tendon (FDS) and suspensory ligament (SL) explants were cultured in 10% plasma in DMEM (control), BMA, PRP, or PP. TGF‐β1 and PDGF‐BB concentrations were determined at 0, 24, and 96 h of culture using ELISA. Quantitative RT‐PCR for collagen types I and III (COL1A1, COL3A1), cartilage oligomeric matrix protein (COMP), decorin, and matrix metalloproteinases‐3 and 13 (MMP‐3, MMP‐13) was performed. TGF‐β1 and PDGF‐BB concentrations were highest in PRP and PP. Growth factor quantity was unchanged in BMA, increased in PRP, and decreased in PP over 4 days. TGF‐β1 and platelet concentrations were positively correlated. Lyophilized PP and PRP resulted in increased COL1A1:COL3A1 ratio, increased COMP, and decreased MMP‐13 expression. BMA resulted in decreased COMP and increased MMP‐3 and MMP‐13 gene expression. Platelet concentration was positively correlated with COL1A1, ratio of COL1A1:COL3A1, and COMP, and negatively correlated with COL3A1, MMP‐13, and MMP‐3. White blood cell concentration was positively correlated with COL3A1, MMP3, and MMP13, and negatively correlated with a ratio of COL1A1:COL3A1, COMP, and decorin. These findings support further in vivo investigation of PRP and PP for treatment of tendonitis and desmitis. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 1033–1042, 2009
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“Compared to all modalities, PRP demonstrated the greatest upregulation of COL1A1, COMP, decorin and MMP3 genes with the simultaneous decrease of COL3A1 and MMP13 gene expression.”
From Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review (2023) · cites this paper as [38]
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“After 4 days of culture of the equine flexor digitorum superficialis tendon in 5 different matrices, McCarrel et al. [38] detected the higher expression of COL1A1, COL1A1:COL3A1 ratio, COMP and MMP3, and the decreased expression of COL3A1, decorin and MMP13 genes in PRP-containing media compared to controls.”
From Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review (2023) · cites this paper as [38]
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Cites
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Treatment of Chronic Elbow Tendinosis with Buffered Platelet-Rich Plasma
Mishra, A. K., & Pavelko, T. (2006) · The American Journal of Sports Medicine · doi:10.1177/0363546506288850 · Cited by 918
Growth factors and wound healing: Biochemical properties of growth factors and their receptors
Bennett, N. T., & Schultz, G. S. (1993) · The American Journal of Surgery · doi:10.1016/s0002-9610(05)80797-4 · Cited by 658
Comparison of Surgically Repaired Achilles Tendon Tears Using Platelet-Rich Fibrin Matrices
Sánchez, M., Anitua, E., Azofra, J., Andı́a, I., Padilla, S., & Mujika, I. (2006) · The American Journal of Sports Medicine · doi:10.1177/0363546506294078 · Cited by 653
Anitua, E., Andı́a, I., Sánchez, M., Azofra, J., Zalduendo, M., de la Fuente, M., Nurden, P., & Nurden, A. T. (2004) · Journal of Orthopaedic Research® · doi:10.1016/j.orthres.2004.08.015 · Cited by 505
Platelet Rich Plasma: Biology and New Technology
Pietrzak, W. S., & Eppley, B. L. (2005) · Journal of Craniofacial Surgery · doi:10.1097/01.scs.0000186454.07097.bf · Cited by 408
Schnabel, L. V., Mohammed, H. O., Miller, B. J., McDermott, W. G., Jacobson, M. S., Santangelo, K. S., & Fortier, L. A. (2007) · Journal of Orthopaedic Research · doi:10.1002/jor.20278 · Cited by 403
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Research works printed but not linked: 1
- 10.1046/j.1423-0410.1998.7520115.x · DOI
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de Vos, R., Weir, A., van Schie, H. T. M., Bierma‐Zeinstra, S., Verhaar, J. A. N., Weinans, H., & Tol, J. L. (2010) · JAMA · doi:10.1001/jama.2009.1986 · Cited by 843
Baldwin, P. C., Li, D. J., Auston, D. A., Mir, H., Yoon, R. S., & Koval, K. J. (2019) · Journal of Orthopaedic Trauma · doi:10.1097/bot.0000000000001420 · Cited by 781
The Role of Growth Factors in Cartilage Repair
Fortier, L. A., Barker, J. U., Strauss, E. J., McCarrel, T. M., & Cole, B. J. (2011) · Clinical Orthopaedics and Related Research · doi:10.1007/s11999-011-1857-3 · Cited by 671
Self-citationPlatelet‐Rich Plasma: A Milieu of Bioactive Factors
Boswell, S. G., Cole, B. J., Sundman, E. A., Karas, V., & Fortier, L. A. (2012) · Arthroscopy The Journal of Arthroscopic and Related Surgery · doi:10.1016/j.arthro.2011.10.018 · Cited by 617
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Metadata
- Authors
- McCarrel, T., & Fortier, L.
- Affiliations
- Cornell University (US)
- Corresponding author
- Fortier, Lisa
- Venue
- Journal of Orthopaedic Research
- Publisher
- Wiley
- Published
- 2009-01-23
- Volume
- 27
- Issue
- 8
- Pages
- 1033-1042
- ISSN
- 0736-0266 (ISSN-L)
- 1554-527X
- OpenAlex
- W2016774778
- Cited by
- 389
- References
- 61
- Citations by year
- 2026: 10 · 2025: 20 · 2024: 19 · 2023: 17 · 2022: 19 · 2021: 24 · 2020: 22 · 2019: 27 · 2018: 26 · 2017: 20 · 2016: 23 · 2015: 32 · 2014: 30 · 2013: 27 · 2012: 34
- FWCI
- 28.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Orthopaedic Research® (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Harry M. Zweig Memorial Fund for Equine Research
- Topics
- Health Sciences › Dentistry › Periodontics › Periodontal Regeneration and Treatments
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
- Health Sciences › Medicine › Surgery › Orthopedic Surgery and Rehabilitation
- Keywords
- platelet-rich plasma, bone marrow aspirate, growth factor release, tendon regeneration, ligament regeneration, flexor digitorum superficialis tendon, suspensory ligament, TGF-β1, PDGF-BB, collagen I and III, cartilage oligomeric matrix protein, decorin, matrix metalloproteinases, tendinitis, desmitis, gene expression
- MeSH terms
- Becaplermin, Animals, Blood Platelets, Bone Marrow, Horses, Ligaments, Platelet-Derived Growth Factor, RNA, Messenger, Tendons, Trehalose, Gene Expression, Matrix Metalloproteinase 3, Proto-Oncogene Proteins c-sis, Collagen Type I, Collagen Type III, Intercellular Signaling Peptides and Proteins, Matrix Metalloproteinase 13, Platelet-Rich Plasma, Transforming Growth Factor beta1
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