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Preferential star strand biogenesis of pre‐miR‐24‐2 targets PKC‐alpha and suppresses cell survival in MCF‐7 breast cancer cells

Martin, Elizabeth C., Elliott, Steven, Rhodes, Lyndsay V., Antoon, James W., Fewell, Claire, Zhu, Yun, Driver, Jennifer L., Jodari‐Karimi, Mona +5 more·2014·Molecular Carcinogenesis
DOI 10.1002/mc.21946PMC PMC4030540
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Abstract

microRNAs (miRNA) are regulators of cellular pathways and alterations of normal miRNA expression levels have been shown to increase tumorigenesis. miR‐24 has been demonstrated as having both tumor suppressive and oncogenic properties depending on cell context. Here, we demonstrate a possible role for pre‐miR‐24‐2 as a tumor suppressor in the MCF‐7 breast cancer cell line through the preferential processing of mature miR‐24‐2* over miR‐24. Specifically, we show that the ectopic expression of miR‐24‐2* in MCF‐7 breast cancer cells results in a suppression of cellular survival both in vivo and in vitro. Notably, the overexpression of miR‐24‐2* results in a dampening of cell survival through the targeted suppression of PKCα. In addition, a similar biological change is observed in vivo where MCF‐7 cells overexpressing pre‐miR‐24‐2 have decreased tumorigenicity and tumor incidence. Taken together our data demonstrate that when overexpressed biogenesis of the pre‐miR‐24‐2 favors miR‐24‐2* in the MCF‐7 breast cancer cell line and suggests a tumor suppressive role for miR‐24‐2* observed through the inhibition of PKCα‐mediated cellular survival. © 2013 Wiley Periodicals, Inc.

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  • miR-24-2* (5p) directly targets PKCα, a factor required for the survival of MCF-7 breast cancer cells.

    “Here, miR‐24‐2* (5p) directly targets PKCα (protein kinase C), a factor that is required for the survival of MCF‐7 breast cancer cells (Martin et al., 2012; Weldon et al., 2005).”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Martin et al., 2012

  • The miR-24-2* (5p) strand is upregulated in MCF-7 breast cancer cell lines compared to control mammary epithelial cells.

    “The miR‐24‐2* (5p) strand is upregulated in MCF‐7 breast cancer cell lines compared to control mammary epithelial cells (Martin et al., 2012), (Table 1).”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Martin et al., 2012

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Authors
Martin, E. C., Elliott, S., Rhodes, L. V., Antoon, J. W., Fewell, C., Zhu, Y., Driver, J. L., Jodari‐Karimi, M., Taylor, C. W., Flemington, E. K., Beckman, B. S., Collins‐Burow, B. M., & Burow, M. E.
Affiliations
  • Department of Medicine Section of Hematology and Medical Oncology Tulane University New Orleans Louisiaina
  • Department of Pharmacology Tulane University New Orleans Louisiaina
  • Department of Pathology Tulane University New Orleans Louisiaina
  • Department of Computer Science University of New Orleans New Orleans Louisiaina
Venue
Molecular Carcinogenesis
Publisher
Wiley
Published
2012-08-21
Volume
53
Issue
1
Pages
38-48
ISSN
  • 0899-1987
  • 1098-2744
DOI
10.1002/mc.21946
PMC
PMC4030540
Cited by
43
References
1
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)

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