Gene expression profiles and bioinformatics analysis of insulin‐like growth factor‐1 promotion of osteogenic differentiation
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Background: Insulin‐like growth factor‐1 (IGF‐1) promotes osteoblast differentiation and mineralization. The objective of this study was to investigate the effects of IGF‐1 on proliferation, mineralization, alkaline phosphatase (ALP) synthesis, and gene expression of osteoblast differentiation in MC3T3‐E1 osteoblasts cells, and to explore gene expression profiling differential genes. Methods: MC3T3‐E1 osteoblasts cells were cultured in medium with or without IGF‐1. The ALP assay was employed to determine the osteoblast mineralization, and Alizarin red S to stain for calcium deposits, which were the indicators of mature osteocytes. The living cell number was assessed by the Cell Counting Kit‐8 method. RNA‐seq analysis was applied to identify genes that were differentially expressed in with or without IGF‐1 as well as genes that varied between these two groups. The expression of osteogenic marker genes was determined by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blot analysis. Result: The cell number of osteoblasts exposed to IGF‐1 at 200 μg/L significantly increased compared with the control group. The ALP activity in IGF‐1‐treated cells was higher than that in the control group. IGF‐1 can increase ALP synthesis in osteoblasts in vitro. RNA‐seq analysis showed that 677 triggered differentially expressed genes by IGF, of which 383 genes were downregulated and 294 genes were upregulated. Gene ontology (GO) analysis showed that IGF‐1 caused a significant change in gene expression patterns. Conclusions: This result suggested that IGF‐1 could probably promote the synthesis of organic matrix and mineralize action of bone. Osteogenic‐related genes ( DMP1, PHEX, SOST, BMP2, RUNX2, OPN, and OCN ) were significantly upregulated both in GO analysis and in pathway analysis to perform qRT‐PCR. Western blot analysis demonstrated that the Notch pathway was highly upregulated in MC3T3‐E1 cells.
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IGF-1 recruits progenitor cells of the osteoblastic lineage and promotes osteoblast differentiation and matrix mineralization by upregulating osteogenesis-related genes DMP1, PHEX, SOST, BMP2, RUNX2, OPN, and OCN.
“IGF-1 recruits progenitor cells of the osteoblastic lineage215 and promotes osteoblast differentiation and matrix mineralization by upregulating the osteogenesis‐related genes DMP1, PHEX, SOST, BMP2, RUNX2, OPN, and OCN.216”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [216]
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References 31 · Cited by 20
- Authors
- Yuan, Y., Duan, R., Wu, B., Huang, W., Zhang, X., Qu, M., Liu, T., & Yu, X.
- Affiliations
- Affiliated Zhongshan Hospital of Dalian University (CN)
- Corresponding author
- Yu, Xiaobing
- Venue
- Molecular Genetics & Genomic Medicine
- Publisher
- Wiley
- Published
- 2019-08-16
- Volume
- 7
- Issue
- 10
- Pages
- e00921-e00921
- ISSN
- 2324-9269 (ISSN-L)
- PMC
- PMC7082822
- OpenAlex
- W2967074347
- Cited by
- 20
- References
- 31
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 4 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 2
- FWCI
- 0.66
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecular Genetics & Genomic Medicine (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Alkaline Phosphatase Research Studies
- Keywords
- insulin-like growth factor I, osteogenic differentiation, osteoblasts, MC3T3-E1 cells, bone mineralization, alkaline phosphatase, cell proliferation, differentially expressed genes, Notch signaling, osteogenic marker genes, Runx2, BMP-2, sclerostin, osteopontin, DMP1, PHEX
- MeSH terms
- Alkaline Phosphatase, Animals, Calcification, Physiologic, Cell Differentiation, Cell Line, Insulin-Like Growth Factor I, Osteoblasts, Cell Proliferation, Mice, Receptors, Notch, Transcriptome
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