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Cited by 90Advanced Neuroimaging Techniques and ApplicationsAdvanced MRI Techniques and ApplicationsNMR spectroscopy and applications

Three-dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain

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Aggarwal, Manisha, Mori, Susumu, Shimogori, Tomomi, Blackshaw, Seth, Zhang, Jiangyang·2010·Magnetic Resonance in Medicine
DOI 10.1002/mrm.22426PMC PMC2915547
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Abstract

Diffusion tensor imaging is gaining increasing importance for anatomical imaging of the developing mouse brain. However, the application of diffusion tensor imaging to mouse brain imaging at microscopic levels is hindered by the limitation on achievable spatial resolution. In this study, fast diffusion tensor microimaging of the mouse brain, based on a diffusion‐weighted gradient and spin echo technique with twin‐navigator echo phase correction, is presented. Compared to echo planar and spin echo acquisition, the diffusion‐weighted gradient and spin echo acquisition resulted in significant reduction in scan time and had minimal image distortion, thereby allowing acquisition at higher spatial resolution. In this study, three‐dimensional diffusion tensor microimaging of the mouse brains at spatial resolutions of 50‐60 μm revealed unprecedented anatomical details. Thin fiber bundles in the adult striatum and white matter tracts in the embryonic day 12 mouse brains were visualized for the first time. The study demonstrated that data acquired using the diffusion tensor microimaging technique allow three‐dimensional mapping of gene expression data and can serve as a platform to study gene expression patterns in the context of neuroanatomy in the developing mouse brain. Magn Reson Med 64:249–261, 2010. © 2010 Wiley‐Liss, Inc.

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  • We used an ultra high field 11.7 T scanner with a 3D DW-GRASE sequence that minimized distortion while allowing for a 12-fold acceleration compared to spin echo imaging.

    “we used an ultra high field 11.7 T scanner with a 3D DW-GRASE sequence that minimized distortion while allowing for a 12-fold acceleration compared to spin echo imaging (Aggarwal et al., 2010).”

    From The role of myelination in measures of white matter integrity: Combination of diffusion tensor imaging and two-photon microscopy of CLARITY intact brains (2017) · cites this paper as Aggarwal et al., 2010

    Materials and Methods (DTI acquisition and Pulse sequence details; page unavailable) describe an 11.7 T instrument and a 3D DW-GRASE sequence. The Results (Comparison of GRASE and spin echo DTI; page unavailable) report no discernible geometric distortion relative to multiple spin echo imaging, but a threefold—not 12-fold—reduction in scan time. (LLM, conf=0.93)

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Cited by

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Authors
Aggarwal, M., Mori, S., Shimogori, T., Blackshaw, S., & Zhang, J.
Affiliations
  • Johns Hopkins University (US)
  • Kennedy Krieger Institute (US)
  • RIKEN Center for Brain Science (JP)
Corresponding author
Aggarwal, Manisha
ORCID
Venue
Magnetic Resonance in Medicine
Publisher
Wiley
Published
2010-06-23
Volume
64
Issue
1
Pages
249-261
ISSN
  • 0740-3194 (ISSN-L)
  • 1522-2594
DOI
10.1002/mrm.22426
PMC
PMC2915547
OpenAlex
W2019925358
Cited by
90
References
1
Citations by year
2026: 2 · 2025: 10 · 2024: 2 · 2023: 7 · 2022: 7 · 2021: 3 · 2020: 9 · 2019: 4 · 2018: 7 · 2017: 2 · 2016: 8 · 2015: 4 · 2014: 7 · 2013: 8 · 2012: 5
FWCI
4.68
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Magnetic Resonance in Medicine (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute on Aging (R01AG020012, R01AG20012-01)
  • National Institute of Neurological Disorders and Stroke (R01NS070909, R21NS065306, R21NS059529)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB003543)
  • National Center for Research Resources (P41RR15241-01, P41RR015241)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
  • Physical Sciences › Physics and Astronomy › Nuclear and High Energy Physics › NMR spectroscopy and applications
Keywords
mouse brain, spin echo, high spatial resolution, striatum, white matter tracts, embryonic brain development, gene expression mapping, neuroanatomy
MeSH terms
Animals, Brain, Chromosome Mapping, Female, Gene Expression Regulation, Mice, Inbred C57BL, Radiography, Homeodomain Proteins, Mice, Diffusion Tensor Imaging

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