The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Towards a human proteome atlas: High‐throughput generation of mono‐specific antibodies for tissue profiling
Abstract
A great need exists for the systematic generation of specific antibodies to explore the human proteome. Here, we show that antibodies specific to human proteins can be generated in a high‐throughput manner involving stringent affinity purification using recombinant protein epitope signature tags (PrESTs) as immunogens and affinity‐ligands. The specificity of the generated affinity reagents, here called mono‐specific antibodies (msAb), were validated with a novel protein microarray assay. The success rate for 464 antibodies generated towards human proteins was more than 90% as judged by the protein array assay. The antibodies were used for parallel profiling of patient biopsies using tissue microarrays generated from 48 human tissues. Comparative analysis with well‐characterized monoclonal antibodies showed identical or similar specificity and expression patterns. The results suggest that a comprehensive atlas containing extensive protein expression and subcellular localization data of the human proteome can be generated in an efficient manner with mono‐specific antibodies.
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Cites
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A subcellular map of the human proteome
Thul, P. J., Åkesson, L., Wiking, M., Mahdessian, D., Geladaki, A., Ait Blal, H., Alm, T., Asplund, A., Björk, L., Breckels, L. M., et al. (2017) · Science · doi:10.1126/science.aal3321 · Cited by 2,813
Self-citationA Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics
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Self-citationThe human protein atlas: a spatial map of the human proteome
Thul, P. J., & Lindskog, C. (2018) · Protein Science · doi:10.1002/pro.3307 · Cited by 1,299
Spatial proteomics: a powerful discovery tool for cell biology
Lundberg, E., & Borner, G. H. H. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0094-y · Cited by 591
Carrier-mediated cellular uptake of pharmaceutical drugs: an exception or the rule?
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Metadata
- Authors
- Nilsson, P., Paavilainen, L., Larsson, K., Ödling, J., Sundberg, M., Andersson, A., Kampf, C., Persson, A., Szigyarto, C. A., Ottosson, J., Björling, E., Hober, S., Wernérus, H., Wester, K., Pontén, F., & Uhlen, M.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2005-11
- Volume
- 5
- Issue
- 17
- Pages
- 4327-4337
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W1964396121
- Cited by
- 265
- References
- 20
- FWCI
- 7.72
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nilsson, P., Paavilainen, L., Larsson, K., Ödling, J., Sundberg, M., Andersson, A., Kampf, C., Persson, A., Szigyarto, C. A., Ottosson, J., Björling, E., Hober, S., Wernérus, H., Wester, K., Pontén, F., & Uhlen, M. (2005). Towards a human proteome atlas: High‐throughput generation of mono‐specific antibodies for tissue profiling. PROTEOMICS. https://doi.org/10.1002/pmic.200500072
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