Shedding & shaving: Disclosure of proteomic expressions on a bacterial face
Abstract
Bacterial surface proteins are critically important in determining the success of bacterial strains in their competition for survival, which makes comprehensive knowledge of the microbial 'face' of high relevance to understand bacterial physiology. Therefore, the aim of this study was to inventorize the proteomic composition of a bacterial surface by gel-free approaches using Bacillus subtilis as a model organism. To this purpose, intact bacteria were incubated with trypsin to cleave surface proteins, after which these 'shaved' proteins were identified by LC-MS/MS analysis of tryptic peptides that were released into the incubation buffer. In parallel, proteins that were released from bacterial cells without trypsin treatment were inventorized and defined as 'shed' proteins. The results showed that peptides of 41 proteins, comprising transmembrane proteins, secretory and lipoproteins, known 'anchorless' surface proteins and ribosomal proteins, were specifically released by shaving. Some of these proteins were also cleaved by trypsin-beads, which are unable to penetrate the bacterial cell wall, indicating a genuine surface-exposed localization. Together this shows that these combined gel-free approaches can reveal novel proteomic decorations on a bacterial face and may provide leads for future studies on protein sorting in B. subtilis and other Gram-positive bacteria.
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EF-G, EF-P, and EF-Ts proteins were identified, implying an exposed localization similar to reports in E. faecalis V583 and B. subtilis.
“Except for EF-Tu, we also identified EF-G, EF-P, and EF-Ts proteins and this implies an exposed localization, similar to the report in E. faecalis V583 (Bøhle et al., 2011) and model B. subtilis (Tjalsma et al., 2008).”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Tjalsma et al., 2008
Ribosomal proteins have been identified not only in the cytoplasm but also on the surface of bacteria, and they may play a key role in mediating bacterial adhesion and colonization.
“Recently, ribosomal proteins have been identified not only in the cytoplasm but also on the surface of bacteria, and they may play a key role in mediating bacterial adhesion and colonization; for example, surface-associated ribosomal proteins have been detected in Streptococcus pseudomallei and Bacillus subtilis 168 (Schaumburg et al., 2004; Severin et al., 2007; Tjalsma”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Tjalsma et al., 2008
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References 38 · Cited by 122
- Authors
- Tjalsma, H., Lambooy, L., Hermans, P. W., & Swinkels, D. W.
- Affiliations
- Radboud University Medical Center (NL)
- Corresponding author
- Tjalsma, Harold
- ORCID
- Swinkels, Dorine W.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2008-03-26
- Volume
- 8
- Issue
- 7
- Pages
- 1415-1428
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W2156127424
- Cited by
- 122
- References
- 38
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 2 · 2022: 8 · 2021: 1 · 2020: 5 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 6 · 2015: 5 · 2014: 16 · 2013: 14 · 2012: 16
- FWCI
- 4.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- PROTEOMICS (publishedVersion)
- PubMed (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- bacterial surface proteins, Bacillus subtilis, protein shedding, cell surface proteome, transmembrane proteins, lipoproteins, secreted proteins, ribosomal proteins, protein sorting, Gram-positive bacteria, gel-free proteomics
- MeSH terms
- Bacillus subtilis, Bacterial Proteins, Chromatography, Liquid, Enzymes, Immobilized, Membrane Proteins, Models, Biological, Trypsin, Proteomics, Tandem Mass Spectrometry
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