Towards comprehensive and quantitative proteomics for diagnosis and therapy of human disease
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Abstract
Given superior analytical features, MS proteomics is well suited for the basic investigation and clinical diagnosis of human disease. Modern MS enables detailed functional characterization of the pathogenic biochemical processes, as achieved by accurate and comprehensive quantification of proteins and their regulatory chemical modifications. Here, we describe how high‐accuracy MS in combination with high‐resolution chromatographic separations can be leveraged to meet these analytical requirements in a mechanism‐focused manner. We review the quantification methods capable of producing accurate measurements of protein abundance and posttranslational modification stoichiometries. We then discuss how experimental design and chromatographic resolution can be leveraged to achieve comprehensive functional characterization of biochemical processes in complex biological proteomes. Finally, we describe current approaches for quantitative analysis of a common functional protein modification: reversible phosphorylation. In all, current instrumentation and methods of high‐resolution chromatography and MS proteomics are poised for immediate translation into improved diagnostic strategies for pediatric and adult diseases.
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Cites
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- Authors
- Cifani, P., & Kentsis, A.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Corresponding author
- Cifani, Paolo
- Kentsis, Alex
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2016-12-21
- Volume
- 17
- Issue
- 1-2
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- PMC
- PMC5243792
- OpenAlex
- W2953036329
- Cited by
- 101
- References
- 6
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 17 · 2023: 14 · 2022: 16 · 2021: 17 · 2020: 10 · 2019: 5 · 2018: 5 · 2017: 8
- FWCI
- 0.62
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- PROTEOMICS (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (submittedVersion)
- Funders
- American-Italian Cancer Foundation
- National Institutes of Health (R21 CA188881, P30 CA008748)
- Alex's Lemonade Stand Foundation for Childhood Cancer
- Gabrielle’s Angel Foundation for Cancer Research
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Keywords
- quantitative proteomics, protein abundance quantification, reversible phosphorylation, high-resolution chromatography, therapeutic biomarkers, childhood diseases
- MeSH terms
- Humans, Protein Processing, Post-Translational, Mass Spectrometry, Proteome, Proteomics
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