New tools for recombinant protein production in Escherichia coli: A 5‐year update
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The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost‐effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.
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E. coli has fast growth rate (doubling time 20 min), low cost, easiness of genetic manipulation, well-known molecular features, good productivity, and simple fermentation process development for manufacturing scale-up, making it an affordable expression system for recombinant protein.
“Given the fast growth rate (doubling time is 20 min), the low cost (the medium is inexpensive), the easiness of genetic manipulation, the well-known molecular features, the good productivity and the simple fermentation process development for manufacturing scale-up, this microorganism represents an affordable expression system for recombinant protein [43,44,45,46].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [43]
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Experimental — accuracy not yet measuredMicroalgal and cyanobacterial biomass can be used to produce media for other valuable heterotrophic bacteria, such as E. coli.
“Microalgal and cyanobacterial biomass can also be used to produce media for other valuable heterotrophic bacteria, such as E. coli , a key cell platform used for generating a multitude of valuable recombinant products (Rosano et al., 2019).”
From Cyanobacteria and microalgae in supporting human habitation on Mars (2022) · cites this paper as Rosano et al., 2019
E. coli is one of the most well-established cell factories for production of recombinant proteins.
“E. coli is one of the most well-established cell factories for the production of recombinant proteins (RPP) [43].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [43]
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References 80 · Cited by 372
- Authors
- Rosano, G. L., Morales, E. S., & Ceccarelli, E. A.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Corresponding author
- Rosano, Germán L.
- Venue
- Protein Science
- Publisher
- Wiley
- Published
- 2019-07
- Volume
- 28
- Issue
- 8
- Pages
- 1412-1422
- ISSN
- 0961-8368 (ISSN-L)
- 1469-896X
- PMC
- PMC6635841
- OpenAlex
- W2952116753
- Cited by
- 372
- References
- 80
- Citations by year
- 2026: 41 · 2025: 41 · 2024: 68 · 2023: 58 · 2022: 68 · 2021: 59 · 2020: 27 · 2019: 8 · 2018: 1
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Protein Science (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Repositorio Hipermedial UNR (Universidad Nacional de Rosario) (publishedVersion, cc-by-nc-nd)
- Conicet (submittedVersion, cc-by-nc-sa)
- Funders
- Fondo para la Investigación Científica y Tecnológica (PICT2015‐2955)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- recombinant protein expression, Escherichia coli, expression plasmids, growth conditions, biologic therapy, expression systems
- MeSH terms
- Escherichia coli, Recombinant Proteins
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