The molecular cloning of 8-epicedrol synthase from Artemisia annua
Abstract
A cDNA library was prepared from Artemisia annua, and a 129-bp fragment was amplified from this library using primers corresponding to sequences conserved in known dicot sesquiterpene synthases. A 1641-bp open reading frame that encoded a predicted protein 35-38% identical to dicot sesquiterpene synthases was cloned using this fragment as a hybridization probe. The gene product expressed in Escherichia coli cyclized farnesyl diphosphate to a 96:4 mixture of (-)8-epicedrol and cedrol. Neither cedrol epimer was detected by GC-MS in an A. annua extract prepared from the same specimen as the cDNA.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A cDNA clone encoding epi-cedrol synthase catalyzes the formation of epi-cedrol from FPP (378).
“A cDNA clone encoding epi-cedrol synthase has been shown to catalyze the formation of the oxygenated sesquiterpene epi-cedrol from FPP (378) [258,259].”
From The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia annua L. (Qinghao) (2010) · cites this paper as [259]
The citation lists haven’t been fetched yet
References 32 · Cited by 48
- Authors
- Hua, L., & Matsuda, S. P. T.
- Affiliations
- Rice University (US)
- Corresponding author
- Matsuda, Seiichi P.T.
- Venue
- Archives of Biochemistry and Biophysics
- Publisher
- Elsevier BV
- Published
- 1999-09
- Volume
- 369
- Issue
- 2
- Pages
- 208-212
- ISSN
- 0003-9861 (ISSN-L)
- OpenAlex
- W2079761963
- Cited by
- 48
- References
- 32
- Citations by year
- 2025: 1 · 2024: 7 · 2023: 1 · 2021: 3 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2013: 2 · 2012: 3
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Archives of Biochemistry and Biophysics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai041598-04)
- National Institute of Allergy and Infectious Diseases (AI 41598)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- Artemisia annua, sesquiterpene synthase, cDNA cloning, cedrol, sesquiterpenes, gene expression in Escherichia coli
- MeSH terms
- Polycyclic Sesquiterpenes, Amino Acid Sequence, Antimalarials, Cloning, Molecular, Escherichia coli, Models, Chemical, Molecular Sequence Data, Plants, Medicinal, Polyisoprenyl Phosphates, Recombinant Proteins, Sesquiterpenes, Terpenes, Gene Library, Genes, Plant, Sequence Homology, Amino Acid, DNA, Complementary, RNA, Plant, Asteraceae, Carbon-Carbon Lyases, Nuclear Magnetic Resonance, Biomolecular, Artemisinins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
