cDNA Cloning of a Novel Amphiphysin Isoform and Tissue-Specific Expression of Its Multiple Splice Variants
Abstract
Amphiphysin, an SH3-domain containing protein concentrated in nerve terminals, is believed to be involved in the synaptic vesicle recycling. We have cloned cDNAs of a novel isoform of amphiphysin (amphiphysin II) by exploiting sequence information for homologous ESTs deposited in databases. At least 9 different subtypes of the isoform with 50-60% amino acid identity to the human amphiphysin were identified by a conventional library screening and PCR amplification of cDNA libraries. Each subtype probably represents a splice variant of a single gene transcript. Analysis of mRNA expression in various tissues by RT-PCR showed that the isoform is ubiquitously distributed. The expression spectrum of the isoform subtypes, however, is significantly different in several tissues examined, suggesting that they are involved in the regulation of endocytic processes that are unique to each cell type.
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Seven brain-specific and two ubiquitous BIN1 isoforms have been reported previously.
“Expression of seven brain-specific and two ubiquitous BIN1 isoforms have been reported previously [4, 6, 7].”
From Predominant expression of Alzheimer’s disease-associated BIN1 in mature oligodendrocytes and localization to white matter tracts (2016) · cites this paper as [7]
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Tissue-specific alternative splicing remodels protein-protein interaction networks
Ellis, J. D., Barrios-Rodiles, M., Çolak, R., Irimia, M., Kim, T., Calarco, J. A., Wang, X., Pan, Q., O'Hanlon, D., Kim, P. M., et al. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.05.037 · Cited by 431 · see citing works
De Rossi, P., Buggia-Prévot, V., Clayton, B. L. L., Vasquez, J. B., van Sanford, C., Andrew, R. J., Lesnick, R., Botté, A., Deyts, C., Salem, S., et al. (2016) · Molecular Neurodegeneration · doi:10.1186/s13024-016-0124-1 · Cited by 151 · see citing works
“Our findings are similar to the previously reported RT-PCR analysis of BIN1 isoform composition in rat brain and peripheral tissue [7].”
Functional coordination of alternative splicing in the mammalian central nervous system
Fagnani, M., Barash, Y., Ip, J. Y., Misquitta, C., Pan, Q., Saltzman, A. L., Shai, O., Lee, L., Rozenhek, A., Mohammad, N., et al. (2007) · Genome Biology · doi:10.1186/gb-2007-8-6-r108 · Cited by 120 · see citing works
- Authors
- Tsutsui, K., Maeda, Y., Tsutsui, K., Seki, S., & Tokunaga, A.
- Affiliations
- Okayama University (JP)
- Corresponding author
- Tsutsui, Ken
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1997-07
- Volume
- 236
- Issue
- 1
- Pages
- 178-183
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1998585742
- Cited by
- 68
- References
- 0
- Citations by year
- 2025: 3 · 2023: 1 · 2020: 1 · 2019: 2 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- amphiphysin, SH3 domain, cDNA cloning, splice variants, protein isoforms, tissue-specific gene expression, synaptic vesicle recycling, endocytosis, presynaptic terminals, ESTs
- MeSH terms
- Amino Acid Sequence, Animals, Cloning, Molecular, Humans, Male, Molecular Sequence Data, Nerve Tissue Proteins, Organ Specificity, RNA Splicing, Sequence Alignment, Rats, Wistar, DNA, Complementary, Rats
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