A Novel Brain-Specific Isoform of β Spectrin: Isolation and Its Interaction with Munc13
Abstract
Munc13 is a component of the neurotransmitter release machinery which is specifically expressed in brain. Munc13 interacts with Doc2 and syntaxin which are also implicated in the neurotransmitter release process. Here we isolated another Munc13-interacting molecule from a rat brain cDNA library by use of the yeast two-hybrid system, identified it to be a novel type of beta spectrin, and named it beta SpIII sigma 1. beta SpIII sigma 1 was specifically expressed in brain, where it was enriched in the synaptic vesicle and plasma membrane fractions. Because spectrin has been shown to interact with the actin cytoskeleton which is involved in the exocytotic process, the present results suggest that the Munc13-beta SpIII sigma 1 interactions play a role in neurotransmitter release.
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Munc13-1 in synapses appears tightly anchored to the active zone via scaffold proteins and interacts with components of the sub-membranous cytoskeleton, rendering it effectively insoluble.
“Although Munc13s do not have transmembrane domains, Munc13-1 in synapses appears to be tightly anchored to the AZ via scaffold proteins [62,63] and interacts with components of the sub-membranous cytoskeleton [64], rendering it effectively insoluble [65].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [64]
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References 35 · Cited by 58
- Authors
- Sakaguchi, G., Orita, S., Naito, A., Maeda, M., Igarashi, H., Sasaki, T., & Takai, Y.
- Affiliations
- Shionogi (Japan) (JP)
- The University of Osaka (JP)
- Corresponding author
- Takai, Yoshimi
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1998-07
- Volume
- 248
- Issue
- 3
- Pages
- 846-851
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1998878692
- Cited by
- 58
- References
- 35
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2017: 1 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 2.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Health, Labour and Welfare
- Human Frontier Science Program
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- beta-spectrin, Munc13, neurotransmitter release, synaptic vesicles, protein-protein interaction, actin cytoskeleton, exocytosis, syntaxin, rat brain
- MeSH terms
- Amino Acid Sequence, Animals, Brain, Cell-Free System, Molecular Sequence Data, Nerve Tissue Proteins, Organ Specificity, Phylogeny, Recombinant Proteins, Spectrin, Blotting, Northern, Gene Library, Sequence Alignment, Conserved Sequence, Sequence Homology, Amino Acid, Rats
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