Inactivation of DNA by β-propiolactone
Abstract
beta-propiolactone (BPL) is an alkylating agent which reacts with many nucleophilic reagents including nucleic acids and proteins. BPL modifies the structure of nucleic acids after reaction mainly with purine residues (notably guanine). It induces nicks in DNA, cross-linking between DNA and proteins as well as between the DNA strands in the double helix. Consequently, BPL is widely used for the inactivation of viruses (DNA and RNA viruses). Moreover, it alters the capability of residual/contaminating cell DNA to be used as template by various polymerases. Thus, BPL reduces the risks associated with residual/contaminating cell DNA in biologicals.
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BPL alters the structure and capability of rcDNA to be used as a template by various polymerases.
“In addition, BPL alters the structure (Kubinski and Szybalski, 1975;Perrin and Morgeaux, 1995) and capability of rcDNA to be used as a template by various polymerases, further reducing the potential associated risk.”
From Comparative analysis of the performance of residual host cell DNA assays for viral vaccines produced in Vero cells (2019) · cites this paper as Perrin and Morgeaux, 1995
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Vernay, O., Sarcey, E., Detrez, V., Abachin, E., Riou, P., Mouterde, B., Bonnevay, T., & Mallet, L. (2019) · Journal of Virological Methods · doi:10.1016/j.jviromet.2019.01.001 · Cited by 14 · see citing works
- Authors
- Perrin, P., & Morgeaux, S.
- Affiliations
- Institut Pasteur (FR)
- Corresponding author
- Perrin, Pierre
- Venue
- Biologicals
- Publisher
- Elsevier BV
- Published
- 1995-09
- Volume
- 23
- Issue
- 3
- Pages
- 207-211
- ISSN
- 1045-1056 (ISSN-L)
- OpenAlex
- W2061772914
- Cited by
- 82
- References
- 0
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 6 · 2021: 4 · 2020: 2 · 2019: 7 · 2018: 3 · 2017: 5 · 2016: 2 · 2015: 5 · 2014: 4 · 2013: 3 · 2012: 3
- FWCI
- 0.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biologicals (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Keywords
- beta-propiolactone, DNA inactivation, alkylating agents, guanine modification, DNA nicking, DNA-protein cross-linking, virus inactivation, biological products
- MeSH terms
- Alkylating Agents, Animals, Cell Line, DNA, DNA, Viral, Nucleic Acid Conformation, Propiolactone, Rabies virus
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