CYTOKINE GENE EXPRESSION IN LIVER FOLLOWING MINOR OR MAJOR HEPATECTOMY IN RAT
Abstract
Interleukin 6 (IL-6), tumour necrosis factor alpha (TNF-alpha), interleukin 1 (IL-1), and transforming growth factors alpha and beta (TGF-alpha and TGF-beta) are important mediators which play a pleiotropic role in both inflammatory and hepatic regeneration processes. It has also been proposed that a major hepatectomy impairs the liver-related host defence mechanisms. The aim of this study was to evaluate the influence of minor (30%) vs major (80%) hepatectomy on cytokines, growth factors and acute-phase proteins both at the protein and mRNA levels in rat. For that purpose, rats were submitted to either 30% or 80% hepatectomy and sacrificed at intervals up to day 14 post-hepatectomy to collect liver and blood samples. Serum levels of IL-6 and acute-phase proteins (APPs) were determined after RNA extraction, cytokine and acute-phase proteins gene expression were evaluated using a quantitative RT-PCR method. The results demonstrate that liver mRNA levels for IL-6 were early unregulated after a 80% resection only, whereas liver mRNA levels for IL-1 slowly increased following 30 or 80% hepatectomy. For TNF-alpha, no significant changes were observed between groups. Growth factor expression differed according to the extent of hepatic resection. Moreover, plasma levels of alpha2-macroglobulin (alpha2M) and alpha1 acid glycoprotein (AGP), two major APPs which respond differently to combination of cytokines, were significantly lowered after a major resection whereas levels of serum I…
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At 6 h after PHx, IL-6 mRNAs are readily apparent across liver tissue, correlating with a general increase in hepatic IL-6 protein and RNA.
“However, in livers we tested at 6 h after PHx, when the circulating levels of IL-6 protein become elevated [14], IL-6 mRNAs were readily apparent across the tissue (Figure 2B), correlating with a general increase in hepatic IL-6 protein and RNA (Figure 2C).”
From Synthesis of IL-6 by Hepatocytes Is a Normal Response to Common Hepatic Stimuli (2014) · cites this paper as [14]
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Synthesis of IL-6 by Hepatocytes Is a Normal Response to Common Hepatic Stimuli
Norris, C. A., He, M., Kang, L.-I., Ding, M. Q., Radder, J. E., Haynes, M. M., Yang, Y., Paranjpe, S., Bowen, W. C., Orr, A., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0096053 · Cited by 132 · see citing works
“In remnant livers removed after 70% PHx, enhanced phosphorylation of MET, the HGF receptor, is detected from 5 to 60 min after resection [12], NF k B translocation is evident as early as 30 min after resection [13], and IL-6 mRNA levels begin to increase by 2 h, remaining elevated for 24 h [14].”
BackgroundMurtha-Lemekhova, A., Fuchs, J., Ghamarnejad, O., Nikdad, M., Probst, P., & Hoffmann, K. (2021) · Scientific Reports · doi:10.1038/s41598-021-92888-4 · Cited by 36 · see citing works
- Authors
- Scotté, M., Masson, S., Lyoumi, S., Hiron, M., Ténière, P., Lebreton, J. P., & Daveau, M.
- Affiliations
- Inserm (FR)
- Corresponding author
- Scotté, Michel
- ORCID
- Scotté, Michel
- Venue
- Cytokine
- Publisher
- Elsevier BV
- Published
- 1997-11
- Volume
- 9
- Issue
- 11
- Pages
- 859-867
- ISSN
- 1043-4666 (ISSN-L)
- OpenAlex
- W2019537601
- Cited by
- 60
- References
- 0
- Citations by year
- 2021: 3 · 2020: 1 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 1
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cytokine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
- Health Sciences › Medicine › Hepatology › Hepatocellular Carcinoma Treatment and Prognosis
- Keywords
- hepatectomy, cytokine gene expression, interleukin-6, TNF-α, interleukin-1, transforming growth factor-alpha, TGF-beta, acute phase proteins, alpha2-macroglobulin, alpha-1-acid glycoprotein, liver regeneration, liver inflammation, host defense
- MeSH terms
- Acute-Phase Proteins, Animals, Growth Substances, Hepatectomy, Interleukin-1, Liver, Liver Regeneration, Male, RNA, Messenger, Tumor Necrosis Factor-alpha, Interleukin-6, Gene Expression, Polymerase Chain Reaction, Cytokines, Transforming Growth Factor alpha, Transforming Growth Factor beta, Rats, Sprague-Dawley, Rats
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