Hormonal Regulation and Patterning of the Broad-Complex in the Epidermis and Wing Discs of the Tobacco Hornworm, Manduca sexta
Open accessAbstract
Expression of Manduca Broad-Complex (BR-C) mRNA in the larval epidermis is under the dual control of ecdysone and juvenile hormone (JH). Immunocytochemistry with antibodies that recognize the core, Z2, and Z4 domains of Manduca BR-C proteins showed that BR-C appearance not only temporally correlates with pupal commitment of the epidermis on day 3 of the fifth (final) larval instar, but also occurs in a strict spatial pattern within the abdominal segment similar to that seen for the loss of sensitivity to JH. Levels of Z2 and Z4 BR-C proteins shift with Z2 predominating at pupal commitment and Z4 dominant during early pupal cuticle synthesis. Both induction of BR-C mRNA in the epidermis by 20-hydroxyecdysone (20E) and its suppression by JH were shown to be independent of new protein synthesis. For suppression JH must be present during the initial exposure to 20E. When JH was given 6 h after 20E, suppression was only seen in those regions that had not yet expressed BR-C. In the wing discs BR-C was first detected earlier 1.5 days after ecdysis, coincident with the pupal commitment of the wing. Our findings suggest that BR-C expression is one of the first molecular events underlying pupal commitment of both epidermis and wing discs.
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In holometabolous insects, a small increase in the expression of 20E in the absence of JH during the final larval instar induces pupal commitment, leading to epidermal expression of the transcription factor broad (br).
“In holometabolous insects, a small increase in the expression of 20E in the absence of JH during the final larval instar induces pupal commitment, leading to epidermal expression of the transcription factor broad ( br ) (Zhou & Riddiford, 2001).”
From Why grow up? <scp>A</scp> perspective on insect strategies to avoid metamorphosis (2016) · cites this paper as Zhou & Riddiford, 2001
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References 47 · Cited by 117
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- Authors
- Zhou, B., & Riddiford, L. M.
- Affiliations
- University of Washington (US)
- Corresponding author
- Riddiford, Lynn M.
- Venue
- Developmental Biology
- Publisher
- Elsevier BV
- Published
- 2001-03
- Volume
- 231
- Issue
- 1
- Pages
- 125-137
- ISSN
- 0012-1606 (ISSN-L)
- OpenAlex
- W2168930581
- Cited by
- 117
- References
- 47
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 3 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 7 · 2018: 3 · 2017: 7 · 2016: 5 · 2015: 6 · 2014: 4 · 2013: 3 · 2012: 6
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM060122, GM 60122)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Broad-Complex, Manduca sexta, ecdysone, juvenile hormone, epidermis, wing disc, Z2, Z4, 20-hydroxyecdysone, hormone regulation of gene expression, cuticle biosynthesis, larval development
- MeSH terms
- Animals, Ecdysone, Epidermis, Juvenile Hormones, Metamorphosis, Biological, RNA, Messenger, Transcription Factors, Wings, Animal, Zinc Fingers, Manduca, Drosophila Proteins
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