Age-related alterations in the activation of heat shock transcription factor 1 in rat hepatocytes
Abstract
The induction of hsp70 transcription by heat shock is significantly reduced in hepatocytes isolated from old rats compared to hepatocytes isolated from young/adult rats, and the decline in hsp70 transcription is correlated with a decrease in the induction of heat shock transcription factor 1 (HSF1) binding to the heat shock element. However, the decreased HSF1 binding activity to DNA is not due to reduced levels of HSF1 that are available for activation by heat shock. In fact, the levels of HSF1 are two- to threefold higher in hepatocytes from old rats, and the age-related increase in the levels of HSF1 protein in hepatocytes appears to arise from a decrease in the degradation of the HSF1 because HSF1 mRNA levels do not change and the synthesis of HSF1 decreases approximately 50% with age. No evidence was found for an impairment in HSF1 oligomerization in hepatocytes from old rats, e.g., the level of HSF1 trimers, the nuclear translocation of HSF1, and the phosphorylation of HSF1 after heat shock are similar in hepatocytes isolated from young/adult and old rats. However, the thermostability of the DNA binding activity of HSF1 was significantly reduced with age in a cell-free system as well as in isolated hepatocytes.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The failure to upregulate chaperone transcription with age, at least in the case of hsp70, is due to the inability of HSF to bind the heat shock element on the chaperone gene promoter.
“The reason behind the failure to upregulate chaperone transcription with age, at least in the case of hsp70, has been narrowed to the inability of HSF to bind the heat shock element on the chaperone gene promoter (Ambra et al., 2004; Heydari et al., 2000; Locke and Tanguay, 1996; Singh et al., 2006).”
From Protein Homeostasis and Aging: the importance of exquisite quality control (2011) · cites this paper as Heydari et al., 2000
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 58 · Cited by 125
- Authors
- Heydari, A. R., You, S., Takahashi, R., Gutsmann-Conrad, A., Sarge, K. D., & Richardson, A.
- Affiliations
- Wayne State University (US)
- The University of Texas at San Antonio Health Science Center (US)
- University of Kentucky (US)
- Corresponding author
- Richardson, Arlan
- Venue
- Experimental Cell Research
- Publisher
- Elsevier BV
- Published
- 2000-04
- Volume
- 256
- Issue
- 1
- Pages
- 83-93
- ISSN
- 0014-4827 (ISSN-L)
- OpenAlex
- W2061962861
- Cited by
- 125
- References
- 58
- Citations by year
- 2026: 1 · 2024: 3 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 6 · 2017: 2 · 2015: 4 · 2014: 6 · 2013: 6 · 2012: 7
- FWCI
- 1.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ag001548-14)
- National Institute on Aging (AG-01188, P01AG001188, AG01548)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
- Keywords
- heat shock transcription factor 1, HSF1, hsp70 transcription, aging, hepatocytes, HSF1 phosphorylation
- MeSH terms
- Heat Shock Transcription Factors, Aging, Animals, Cell Nucleus, Cells, Cultured, DNA-Binding Proteins, Drug Stability, Hot Temperature, Heat-Shock Proteins, Kinetics, Liver, Male, Rats, Inbred F344, Transcription Factors, Transcription, Genetic, Macromolecular Substances, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
