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Characterization of the Gene EPAC2: Structure, Chromosomal Localization, Tissue Expression, and Identification of the Liver-Specific Isoform

Ueno, Hiroaki, Shibasaki, Tadao, Iwanaga, Toshihiko, Takahashi, Kazuo, Yokoyama, Yuichi, Liu, Li-Mei, Yokoi, Norihide, Ozaki, Nobuaki, Matsukura, Shigeru, Yano, Hideki, Seino, Susumu·2001·Genomics
DOI 10.1006/geno.2001.6641
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Abstract

The liver-specific protein cAMP-GEFII (also known as Epac2) belongs to a family of cyclic adenosine monophosphate (cAMP) binding proteins having guanine nucleotide exchange factor (GEF) activity (the cAMP-GEF family). Here we clone the gene EPAC2, encoding cAMP-GEFII, from a human liver cDNA library. Human EPAC2 has at least 31 exons and is mapped to human chromosome 2q31. Analyses by primer extension, reverse transcriptase-polymerase chain reaction, and in situ hybridization revealed the presence of three transcription start sites of liver-specific Epac2: two major sites located in exon 10 and a minor site in intron 9. The same translation start site is used in all three transcripts. Liver-specific cAMP-GEFII protein, which lacks the first cAMP-binding domain and the Dishevelled/Egl-10/Pleckstrin domain, was detected at 79 kDa by immunoblot analysis, confirming the presence of the short form of cAMP-GEFII in the liver. Liver-specific cAMP-GEFII also has GEF activity toward Rap1. These results demonstrate the presence of liver-specific cAMP-GEFII. Together with the previous finding that cAMP-GEFII is responsible for cAMP-dependent exocytosis in secretory cells, our study suggests that cAMP-GEFII may have a distinct role in liver.

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Research works printed but not linked: 25

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Metadata

Authors
Ueno, H., Shibasaki, T., Iwanaga, T., Takahashi, K., Yokoyama, Y., Liu, L.-M., Yokoi, N., Ozaki, N., Matsukura, S., Yano, H., & Seino, S.
Venue
Genomics
Publisher
Elsevier BV
Published
2001-11
Volume
78
Issue
1-2
Pages
91-98
ISSN
0888-7543
DOI
10.1006/geno.2001.6641
Cited by
58
References
0
Language
en
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