Three-dimensional Reconstruction of theEscherichia coli30 S Ribosomal Subunit in Ice
Abstract
Three-dimensional (3D) reconstructions of both the heat-activated and non-activated 30 S subunit of the Escherichia coli 70 S ribosome were obtained from a frozen hydrated specimen preparation at 1/37 A-1 resolution. Well-characterized features that can be identified in both reconstructions are the head, the base, the platform and the cleft formed between the head and the platform. Comparisons between the 3D maps of 30 S subunits at 0 degree C, heat-activated at 37 degrees C, and the 30 S subunit portion identified in the cryo-3D map of 70 S ribosome reveal conformational changes the subunit probably undergoes during inactive-active transition and upon association with the 50 S subunit. These comparisons also allow us to localize the sites of association of 30 S and 50 S subunits.
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The platform of the 30S is known to change conformation during subunit association.
“Furthermore, the platform of the 30S is known to change conformation during subunit association (Lata et al. 1996”
From The Structural Basis for the Action of the Antibiotics Tetracycline, Pactamycin, and Hygromycin B on the 30S Ribosomal Subunit (2000) · cites this paper as Lata et al. 1996
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Brodersen, D. E., Clemons, W. M., Carter, A. P., Morgan-Warren, R. J., Wimberly, B. T., & Ramakrishnan, V. (2000) · Cell · doi:10.1016/s0092-8674(00)00216-6 · Cited by 950 · see citing works
“Furthermore, the plat-of overcoming microbial resistance. form of the 30S is known to change conformation during subunit association (Lata et al.,1996).”
BackgroundSpahn, C. M. T., Blaha, G., Agrawal, R. K., Penczek, P., Grassucci, R. A., Trieber, C. A., Connell, S. R., Taylor, D. E., Nierhaus, K. H., & Frank, J. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00238-6 · Cited by 81 · see citing works
Self-citation
- Authors
- Lata, K. R., Agrawal, R. K., Penczek, P., Grassucci, R., Zhu, J., & Frank, J.
- Affiliations
- New York State Department of Health (US)
- Corresponding author
- Frank, Joachim
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1996-09
- Volume
- 262
- Issue
- 1
- Pages
- 43-52
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W2134027771
- Cited by
- 99
- References
- 0
- Citations by year
- 2019: 1 · 2014: 6 · 2013: 1 · 2012: 2
- FWCI
- 4.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health
- National Institute of General Medical Sciences (1R 01 GM29169, R01GM029169)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Keywords
- 30S ribosomal subunit, Escherichia coli ribosome, 70S ribosome, 50S subunit, thermal activation, conformational change, subunit association, ribosome structure, cryo-electron tomography
- MeSH terms
- Escherichia coli, Hot Temperature, Ice, Microscopy, Electron, Ribosomes, Molecular Structure
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