Cited by 358Wound Healing and TreatmentsProtease and Inhibitor MechanismsPineapple and bromelain studies

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MMP-8 Is the Predominant Collagenase in Healing Wounds and Nonhealing Ulcers

Nwomeh, Benedict C., Liang, Hui-Xiu, Cohen, I.Kelman, Yager, Dorne R.·1999·Journal of Surgical Research

DOI 10.1006/jsre.1998.5495

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Abstract

Background The initial cleavage of collagen by collagenase represents the rate-limiting step in the degradation of this central extracellular matrix protein. Chronic nonhealing ulcers, especially pressure ulcers, typically contain elevated levels of collagenolytic activity. However, there have been no detailed attempts to identify the source of these collagenases and their activity either in normal healing wounds or in chronic nonhealing ulcers.Materials and methods Levels of the matrix metalloproteinases, MMP-1 and MMP-8, and the tissue inhibitor of matrix metalloproteinases, TIMP-1, were measured in fluids and tissues of healing human wounds and nonhealing ulcers by ELISA. Relative MMP-1 and MMP-8 levels were also analyzed by substrate preference in a functional assay.Results The patterns of the collagenases MMP-1 and MMP-8 in healing wounds were distinct, with MMP-8 appearing in significantly greater amounts than MMP-1. Chronic nonhealing ulcers were characterized by significantly higher levels of MMP-1 and MMP-8, and lower levels of TIMP-1, than in healing wounds. Levels of both MMP-1 and MMP-8 varied greatly in chronic ulcers, although MMP-8 was always the predominant collagenase present in these wounds. Interestingly, these collagenases were present almost exclusively in their inactive forms in healing wounds, whereas nonhealing ulcers possessed significant levels of the active forms of these enzymes.Conclusions These results clearly demonstrate that the neutrophil-derived MMP-8 is the predominant collagenase present in normal healing wounds and suggest that overexpression and activation of this collagenase may be involved in the pathogenesis of nonhealing chronic ulcers. In addition, excessive collagenolytic activity in chronic ulcers is made possible, partly because of the reduced levels of the inhibitor, TIMP-1.

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The physiology of wound bed preparation
Schultz, G. S. (2012) · CRC Press eBooks · doi:10.3109/9781841849270-4 · Cited by 4
Principles of Reconstructive and Plastic Surgery
Bailey, J. V. (2006) · Elsevier eBooks · doi:10.1016/b1-41-600123-9/50026-7 · Cited by 4
A Complete and Versatile Protocol: Decoration of Cell-Derived Matrices with Mass-Encoded Peptides for Multiplexed Protease Activity Detection
Dodt, K., Drießen, M. D., Lamer, S., Schlösser, A., Lühmann, T. C., & Meinel, L. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c01134 · Cited by 4
Treatment of Pressure Ulcers with Noncontact Normothermic Wound Therapy
Whitney, J. D., Salvadalena, G., Higa, L., et al. (2001) · Journal of Wound Ostomy and Continence Nursing · doi:10.1097/00152192-200109000-00007 · Cited by 4
Peptides and Wound Healing: From Monomer to Combination
Liu, C., Yang, Q., & Zhou, Y. L. (2024) · International Journal of Peptide Research and Therapeutics · doi:10.1007/s10989-024-10627-5 · Cited by 3
“They are able to degrade collagen and growth factors that can promote wound healing to cause wound healing abnormalities (Lobmann et al. 2005, 2002; Nwomeh et al. 1999; Sun et al. 2023; Ongenae et al. 2000).”
Human fingertip regeneration follows clinical phases with distinct proteomic signatures
Schultz, J., Patel, P. A., Aires, R., et al. (2025) · npj Regenerative Medicine · doi:10.1038/s41536-025-00441-y · Cited by 3
“A detailed description of chemicals, instrumentation, and software parameters are provided in Tables 3 – 5.”
Matrix metalloproteinase inhibition and antibiotics in the treatment of chronic wounds
Naidoo, C. C. (2009) · Cited by 3
Wounds, Enzymes,and Proteases
Percival, S., & Cutting, K. (2010) · CRC Press eBooks · doi:10.1201/9781420079944-13 · Cited by 3
Advanced acuity in microbial biofilm genesis, development, associated clinical infections and control
Kumar, P., Mishra, S., & Singh, S. (2017) · Journal des Anti-infectieux · doi:10.1016/j.antinf.2017.01.002 · Cited by 3
Wounds, Enzymes, and Proteases
Percival, S. L., & Cochrane, C. A. (2010) · CRC Press eBooks · doi:10.1201/9781420079944-c9 · Cited by 3
Matrix Metalloproteinase 14 in Corneal Neovascularization
Qin, K., Setter, N. W., Yu, L., Vasi, Z., Wu, W., Hwang, Y., Lee, H., Chang, J., Rosenblatt, M. I., Han, K., et al. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27042027 · Cited by 2
Impact of the local application of collagen on the activity of reparative processes in the lower extremity soft tissue of patients with diabetic foot syndrome
Tokmakova, A. Y., Zaytseva, E. L., Doronina, L. P., et al. (2015) · Terapevticheskii arkhiv · doi:10.17116/terarkh2015871072-79 · Cited by 2
Scarless Wound Healing
Moore, A. L., Marshall, C. D., Nauta, A., Lorenz, H. P., & Longaker, M. T. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809880-6.00005-9 · Cited by 2
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“However, unregulated expression of MMP-8 is associated with chronic leg ulcers (Armstrong and Jude, 2002; Nwomeh et al., 1999).”
Background
Strategy of Surgical Management of Peripheral Neuropathy Form of Diabetic Foot Syndrome in Ghana
Rdeini, W. M., Agbenorku, P., & Mitish, V. A. (2014) · Plastic Surgery International · doi:10.1155/2014/185023 · Cited by 2
“It helped to reduce the rate of infection and provided an ideal healing environment by converting chronic wounds into acute; it also helped to reduce chronic inflammatory by-products as demonstrated by other authors in their studies [33–35].”
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Zhao, R., Jackson, C., & Xue, M. (2018) · Recent clinical techniques, results, and research in wounds · doi:10.1007/15695_2018_132 · Cited by 2
The Eye and Foot in Diabetes
Grigsby, J. G., & Derbel, F. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.83159 · Cited by 2
Preparation of the Wound Bed of the Diabetic Foot Ulcer
Falanga, V. (2007) · Humana Press eBooks · doi:10.1007/978-1-59745-075-1_15 · Cited by 2
“In contrast, it has been suggested that excessive activity (or maldistribution) of other enzymes (MMP-2, MMP-8, MMP-9) contribute to healing impairment (30, 31 ).”
Background
Biological Activity and Implications of the Metalloproteinases in Diabetic Foot Ulcers
la Rosa, C. C.-D., Veloz, I. G., Cardenas‐Vargas, E., Castañeda-Miranda, R., Solís-Sánchez, L. O., Ortiz-Rodriguez, J. M., Vega-Carrillo, H. R., Martínez-Blanco, M. D. R., Flores‐Morales, V., Hernandez-Delgadillo, G. P., et al. (2017) · InTech eBooks · doi:10.5772/intechopen.71725 · Cited by 2
“Chronic wounds, including venous leg ulcers (VLUs) [40, 61, 62], DFUs [63, 64], PUs [65] dehiscent surgical wounds and acute wounds that have become chronic, were found to have elevated MMP activity.”
Background
Matrix Metalloproteinases and their Tissue Inhibitors as Biomarkers in Ulcerative Colitis and Crohn s Disease
Mäkitalo, L. (2010) · Työväentutkimus Vuosikirja · Cited by 2
Non-invasive optical technologies to monitor wound healing
Zhu, L., & Papazoglou, E. S. (2007) · doi:10.17918/etd-2757 · Cited by 2
Current Trends in the Development of Wound Dressings, Biomaterials and Devices
Banerjee, A., & Bhattacharyya, J. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-820352-1.00117-6 · Cited by 2

Authors: Nwomeh, B. C., Liang, H.-X., Cohen, I. K., & Yager, D. R.
Affiliations: Virginia Commonwealth University (US)
ORCID: Nwomeh, Benedict C.
Venue: Journal of Surgical Research
Publisher: Elsevier BV
Published: 1999-02
Volume: 81
Issue: 2
Pages: 189-195
ISSN: 0022-4804 (ISSN-L)
DOI: 10.1006/jsre.1998.5495
OpenAlex: W1988325190
Cited by: 357
References: 0
Citations by year: 2026: 6 · 2025: 9 · 2024: 14 · 2023: 8 · 2022: 8 · 2021: 11 · 2020: 13 · 2019: 10 · 2018: 18 · 2017: 18 · 2016: 18 · 2015: 14 · 2014: 9 · 2013: 15 · 2012: 20
FWCI: 12.03
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Journal of Surgical Research (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Institute of General Medical Sciences (GM20298)
Topics: Health Sciences › Medicine › Surgery › Wound Healing and Treatments
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pineapple and bromelain studies
Keywords: MMP-8, MMP-1, TIMP-1, collagenase, wound healing, chronic non-healing ulcers, pressure ulcers, collagen degradation, extracellular matrix, neutrophils, matrix metalloproteinases, collagenolytic activity
MeSH terms: Adult, Chronic Disease, Pressure Ulcer, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Mastectomy, Middle Aged, Skin, Surgical Flaps, Time Factors, Varicose Ulcer, Wound Healing, Wounds and Injuries, Collagenases, Tissue Inhibitor of Metalloproteinase-1, Matrix Metalloproteinase 1, Matrix Metalloproteinase 8

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MMP-8 Is the Predominant Collagenase in Healing Wounds and Nonhealing Ulcers

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