Cited by 559RNA and protein synthesis mechanismsRNA Interference and Gene DeliveryRNA Research and Splicing

The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.

IRES-Dependent Second Gene Expression Is Significantly Lower Than Cap-Dependent First Gene Expression in a Bicistronic Vector

Open access

Mizuguchi, Hiroyuki, Xu, Zhili, Ishii-Watabe, Akiko, Uchida, Eriko, Hayakawa, Takao·2000·Molecular Therapy

DOI 10.1006/mthe.2000.0050

Open this paper in CoChat — free account Open PDF

Abstract

The internal ribosome entry site (IRES) has been widely used to coexpress heterologous gene products by a message from a single promoter. However, little is known about the efficiency of IRES-dependent second gene expression in comparison with that of first gene expression. This study was undertaken to characterize the relative expression of IRES-dependent second gene in a bicistronic vector, which was derived from the 5' untranslated regions of the encephalomyocarditis virus (EMCV). IRES-dependent second gene expression was compared with cap-dependent first gene expression in several cultured cell lines and in mouse liver in vivo. The expression of the IRES-dependent second gene ranged from 6 to 100% (in most cases between 20 and 50%) that of the first gene. Second gene expression in a plasmid without the IRES was 0.1-0.8% (with some exceptions) that of the first gene. These findings have important implications for the use of IRES, i.e., care should be taken regarding the decreased capacity of IRES-dependent downstream gene expression as well as in determining which gene should be positioned as the first or second gene in a bicistronic vector.

Integrity signals

Registry

Crossref · 10.1006/mthe.2000.0050

Cites

36 of 36 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Efficient selection for high-expression transfectants with a novel eukaryotic vector
Niwa, H., Yamamura, K., & Miyazaki, J. (1991) · Gene · doi:10.1016/0378-1119(91)90434-d · Cited by 5,149
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes
Kozak, M. (1986) · Cell · doi:10.1016/0092-8674(86)90762-2 · Cited by 4,741
The scanning model for translation: an update.
Kozak, M. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.2.229 · Cited by 3,485
Improved retroviral vectors for gene transfer and expression.
Miller, A. D., & Rosman, G. J. (1989) · PubMed · Cited by 1,990
Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA
Pelletier, J., & Sonenberg, N. (1988) · Nature · doi:10.1038/334320a0 · Cited by 1,953
Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA
Liu, F., Song, Y. K., & Liu, D. (1999) · Gene Therapy · doi:10.1038/sj.gt.3300947 · Cited by 1,728
A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation
Jang, S. K., Kräusslich, H., Nicklin, M. J. H., Duke, G. M., Palmenberg, A. C., & Wimmer, E. (1988) · Journal of Virology · doi:10.1128/jvi.62.8.2636-2643.1988 · Cited by 1,390
Effects of Intercistronic Length on the Efficiency of Reinitiation by Eucaryotic Ribosomes
Kozak, M. (1987) · Molecular and Cellular Biology · doi:10.1128/mcb.7.10.3438-3445.1987 · Cited by 537
New mammalian expression vectors
Moss, B., Elroy‐Stein, O., Mizukami, T., Alexander, W. A., & Fuerst, T. R. (1990) · Nature · doi:10.1038/348091a0 · Cited by 523
Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism
Emerman, M., & Temin, H. M. (1984) · Cell · doi:10.1016/0092-8674(84)90453-7 · Cited by 434

Show all 34 references

Sorted by citations

Engineering strategies to overcome the current roadblocks in CAR T cell therapy
Rafiq, S., Hackett, C. S., & Brentjens, R. J. (2019) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-019-0297-y · Cited by 1,525
Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide–based retroviral vector
Szymczak, A. L., Workman, C. J., Wang, Y., Vignali, K. M., Dilioglou, S., Vanin, E. F., & Vignali, D. A. A. (2004) · Nature Biotechnology · doi:10.1038/nbt957 · Cited by 1,059
Stochastic Gene Expression in a Lentiviral Positive-Feedback Loop: HIV-1 Tat Fluctuations Drive Phenotypic Diversity
Weinberger, L. S., Burnett, J., Toettcher, J. E., Arkin, A. P., & Schaffer, D. V. (2005) · Cell · doi:10.1016/j.cell.2005.06.006 · Cited by 633
The Heteromeric Organic Solute Transporter α-β, Ostα-Ostβ, Is an Ileal Basolateral Bile Acid Transporter
Dawson, P. A., Hubbert, M. L., Haywood, J., Craddock, A. L., Zerangue, N., Christian, W. V., & Ballatori, N. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m412752200 · Cited by 381
Stable antibody expression at therapeutic levels using the 2A peptide
Fang, J., Qian, J., Yi, S., Harding, T. C., Tu, G. H., VanRoey, M. J., & Jooss, K. U. (2005) · Nature Biotechnology · doi:10.1038/nbt1087 · Cited by 370
Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters
Amendola, M., Venneri, M. A., Biffi, A., Vigna, E., & Naldini, L. (2004) · Nature Biotechnology · doi:10.1038/nbt1049 · Cited by 324
Immunogenicity of CAR T cells in cancer therapy
Wagner, D. L., Fritsche, E., Pulsipher, M. A., Ahmed, N. M., Hamieh, M., Hegde, M. G., Ruella, M., Savoldo, B., Shah, N. N., Turtle, C. J., et al. (2021) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-021-00476-2 · Cited by 318
Design and generation of recombinant rabies virus vectors
Osakada, F., & Callaway, E. M. (2013) · Nature Protocols · doi:10.1038/nprot.2013.094 · Cited by 298
Development of 2A peptide-based strategies in the design of multicistronic vectors
Szymczak-Workman, A. L., & Vignali, D. A. A. (2005) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.5.5.627 · Cited by 283
AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells
Leaver, S. G., Cui, Q., Plant, G. W., Arulpragasam, A., Hisheh, S., Verhaagen, J., & Harvey, A. R. (2006) · Gene Therapy · doi:10.1038/sj.gt.3302791 · Cited by 274
TRPV1 channels are critical brain inflammation detectors and neuropathic pain biomarkers in mice
Marrone, M. C., Morabito, A., Giustizieri, M., Chiurchiù, V., Leuti, A., Mattioli, M., Marinelli, S., Riganti, L., Lombardi, M., Murana, E., et al. (2017) · Nature Communications · doi:10.1038/ncomms15292 · Cited by 250
Selection methods for high-producing mammalian cell lines
Browne, S. M., & Al‐Rubeai, M. (2007) · Trends in biotechnology · doi:10.1016/j.tibtech.2007.07.002 · Cited by 241
Intraclonal Protein Expression Heterogeneity in Recombinant CHO Cells
Pilbrough, W., Munro, T. P., & Gray, P. P. (2009) · PLoS ONE · doi:10.1371/journal.pone.0008432 · Cited by 210
Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos
Provost, E., Rhee, J. M., & Leach, S. D. (2007) · genesis · doi:10.1002/dvg.20338 · Cited by 190
Translational Efficiency of EMCV IRES in Bicistronic Vectors is Dependent Upon IRES Sequence and Gene Location
Bochkov, Y. A., & Palmenberg, A. C. (2006) · BioTechniques · doi:10.2144/000112243 · Cited by 188
Labeling proteins on live mammalian cells using click chemistry
Nikić, I., Kang, J. H., Girona, G. E., Aramburu, I. V., & Lemke, E. A. (2015) · Nature Protocols · doi:10.1038/nprot.2015.045 · Cited by 181
Promoter trapping reveals significant differences in integration site selection between MLV and HIV vectors in primary hematopoietic cells
Palma, M. D., Montini, E., de Sio, F. R. S., Benedicenti, F., Gentile, A., Médico, E., & Naldini, L. (2004) · Blood · doi:10.1182/blood-2004-03-0798 · Cited by 174
AAV-Mediated Delivery of Ciliary Neurotrophic Factor Prolongs Photoreceptor Survival in the Rhodopsin Knockout Mouse
Liang, F.-Q., Dejneka, N. S., Cohen, D. R., Krasnoperova, N. V., Lem, J., Maguire, A. M., Dudus, L., Fisher, K. J., & Bennett, J. (2001) · Molecular Therapy · doi:10.1006/mthe.2000.0252 · Cited by 172
Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells
Yu, X., Zhan, X., D’Costa, J., Tanavde, V., Ye, Z., Peng, T., Malehorn, M., Yang, X., Civin, C. I., & Cheng, L. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00104-7 · Cited by 166
Codon modification of T cell receptors allows enhanced functional expression in transgenic human T cells
Scholten, K. B. J., Kramer, D., Taanman-Kueter, E. W., Graf, M., Schoedl, T., Meijer, C. J. L. M., Schreurs, M. W. J., & Hooijberg, E. (2006) · Clinical Immunology · doi:10.1016/j.clim.2005.12.009 · Cited by 164
A hepatocellular carcinoma-specific adenovirus variant, CV890, eliminates distant human liver tumors in combination with doxorubicin.
Li, Y., Yu, D., Chen, Y., Amin, P. R., Zhang, H., Nguyen, N., & Henderson, D. (2001) · PubMed · Cited by 147
Highly Efficient Multicistronic Lentiviral Vectors with Peptide 2A Sequences
Ibrahimi, A., Velde, G. V., Reumers, V., Toelen, J., Thiry, I., Vandeputte, C., Vets, S., Deroose, C. M., Bormans, G. M., Baekelandt, V., et al. (2009) · Human Gene Therapy · doi:10.1089/hum.2008.188 · Cited by 145
Nanomedicine-Based Approaches for mRNA Delivery
Uchida, S., Perche, F., Pichon, C., & Cabral, H. (2020) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.0c00618 · Cited by 139
Clinical delivery of circular RNA: Lessons learned from RNA drug development
Young, T. L., Wang, K. C., Varley, A., & Li, B. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114826 · Cited by 130
Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro
Lesueur, L. L., Mir, L. M., & André, F. M. (2016) · Molecular Therapy - Nucleic Acids · doi:10.1038/mtna.2016.4 · Cited by 118
Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNA i Met Ternary Complex Availability
Robert, F., Kapp, L. D., Khan, S. N., Acker, M. G., Kolitz, S. E., Kazemi, S., Kaufman, R. J., Merrick, W. C., Koromilas, A. E., Lorsch, J. R., et al. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-06-0478 · Cited by 121
Enhanced functionality of T cell receptor-redirected T cells is defined by the transgene cassette
Leisegang, M., Engels, B., Meyerhuber, P., Kieback, E., Sommermeyer, D., Xue, S., Reuß, S., Stauss, H. J., & Uckert, W. (2008) · Journal of Molecular Medicine · doi:10.1007/s00109-008-0317-3 · Cited by 121
Adenovirus vectors containing chimeric type 5 and type 35 fiber proteins exhibit altered and expanded tropism and increase the size limit of foreign genes
Mizuguchi, H., & Hayakawa, T. (2002) · Gene · doi:10.1016/s0378-1119(02)00410-9 · Cited by 118
Self-citation
Generation of stable Drosophila cell lines using multicistronic vectors
González-Lopez, M., Martín‐Ruiz, I., Jiménez, S., Pirone, L., Barrio, R., & Sutherland, J. D. (2011) · Scientific Reports · doi:10.1038/srep00075 · Cited by 117
Genetic Modification of Human Embryonic Stem Cells for Derivation of Target Cells
Giudice, A., & Trounson, A. O. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.04.003 · Cited by 115
A Nanobody-Based System Using Fluorescent Proteins as Scaffolds for Cell-Specific Gene Manipulation
Tang, J. C. Y., Szikra, T., Kozorovitskiy, Y., Teixiera, M., Sabatini, B. L., Roska, B., & Cepko, C. L. (2013) · Cell · doi:10.1016/j.cell.2013.07.021 · Cited by 112
An Ion Transport-Independent Role for the Cation-Chloride Cotransporter KCC2 in Dendritic Spinogenesis In Vivo
Fiumelli, H., Briner, A., Puskarjov, M., Blaesse, P., Belem, B. J., Dayer, A. G., Kaila, K., Martin, J., & Vutskits, L. (2012) · Cerebral Cortex · doi:10.1093/cercor/bhs027 · Cited by 126
Two‐promoter vector is highly efficient for overproduction of protein complexes
Kim, K., Kim, H.-E., Lee, K., Han, W.-D., Yi, M., Jeong, J., & Oh, B. (2004) · Protein Science · doi:10.1110/ps.04644504 · Cited by 113
Gene therapy and transplantation in CNS repair: The visual system
Harvey, A. R., Hu, Y. J., Leaver, S. G., Mellough, C., Park, K. K., Verhaagen, J., Plant, G. W., & Cui, Q. (2006) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2006.07.002 · Cited by 110
Skipping the co-expression problem: the new 2A "CHYSEL" technology
de Felipe, P. (2004) · Genetic Vaccines and Therapy · doi:10.1186/1479-0556-2-13 · Cited by 110
Parkin Gene Therapy for α-Synucleinopathy: A Rat Model of Parkinson's Disease
Yamada, M., Mizuno, Y., & Mochizuki, H. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.262 · Cited by 111
Krox-20 patterns the hindbrain through both cell-autonomous and non cell-autonomous mechanismsPreprint
Giudicelli, F., Taillebourg, E., Charnay, P., & Gilardi‐Hebenstreit, P. (2001) · Genes & Development · doi:10.1101/gad.189801 · Cited by 107
Engineered Human tmpk/AZT As a Novel Enzyme/Prodrug Axis for Suicide Gene Therapy
Sato, T., Neschadim, A., Konrad, M., Fowler, D. H., Lavie, A., & Medin, J. A. (2007) · Molecular Therapy · doi:10.1038/mt.sj.6300122 · Cited by 105
Novel insights into the expression pattern of anaphylatoxin receptors in mice and men
Laumonnier, Y., Karsten, C. M., & Köhl, J. (2017) · Molecular Immunology · doi:10.1016/j.molimm.2017.05.019 · Cited by 102
CAR-T cell potency: from structural elements to vector backbone components
Mazinani, M., & Rahbarizadeh, F. (2022) · Biomarker Research · doi:10.1186/s40364-022-00417-w · Cited by 111
Polycistronic Viral Vectors
de Felipe, P. (2002) · Current Gene Therapy · doi:10.2174/1566523023347742 · Cited by 100
Recombinant AAV vectors containing the foot and mouth disease virus 2A sequence confer efficient bicistronic gene expression in cultured cells and rat substantia nigra neurons
Furler, S., Paterna, J. C., Weibel, M., & Büeler, H. (2001) · Gene Therapy · doi:10.1038/sj.gt.3301469 · Cited by 103
MicroRNAs and Cardiac Regeneration
Hodgkinson, C. P., Kang, M. H., Dal-Pra, S., Mirotsou, M., & Dzau, V. J. (2015) · Circulation Research · doi:10.1161/circresaha.116.304377 · Cited by 99
T Cell Receptor Gene Therapy for Cancer
Schmitt, T. M., Ragnarsson, G. B., & Greenberg, P. D. (2009) · Human Gene Therapy · doi:10.1089/hum.2009.146 · Cited by 98
Intratumoral Delivery of p2CMVmIL-12 Using Water-Soluble Lipopolymers
Mahato, R. I., Lee, M., Han, S.-O., Maheshwari, A., & Kim, S. W. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0425 · Cited by 94
Lentiviral Expression of Retinal Guanylate Cyclase-1 (RetGC1) Restores Vision in an Avian Model of Childhood Blindness
Williams, M. L., Coleman, J. E., Haire, S. E., Alemán, T. S., Cideciyan, A. V., Sokal, I., Palczewski, K., Jacobson, S. G., & Semple‐Rowland, S. L. (2006) · PLoS Medicine · doi:10.1371/journal.pmed.0030201 · Cited by 91
Exploiting Internal Ribosome Entry Sites in Gene Therapy Vector Design
Ngoi, S.-M., Chien, A., & Lee, C. G. L. (2004) · Current Gene Therapy · doi:10.2174/1566523044578095 · Cited by 87
Recognition of NY-ESO-1+ tumor cells by engineered lymphocytes is enhanced by improved vector design and epigenetic modulation of tumor antigen expression
Wargo, J. A., Robbins, P. F., Li, Y., Zhao, Y., El‐Gamil, M., Caragacianu, D. L., Zheng, Z., Hong, J. A., Downey, S. G., Schrump, D. S., et al. (2008) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-008-0562-x · Cited by 87
Accelerated Clone Selection for Recombinant CHO Cells Using a FACS‐Based High‐Throughput Screen
DeMaria, C. T., Cairns, V. R., Schwarz, C., Zhang, J., Guerin, M., Zuena, E., Estes, S. D., & Karey, K. P. (2007) · Biotechnology Progress · doi:10.1021/bp060298i · Cited by 86
Approaches to Enhance the Efficacy of DNA Vaccines
Manoj, S., Babiuk, L. A., & den Hurk, S. V. D. L. (2004) · Critical Reviews in Clinical Laboratory Sciences · doi:10.1080/10408360490269251 · Cited by 84
Construction of an rtTA2s-m2/ttskid-Based transcription regulatory switch that displays no basal activity, good inducibility, and high responsiveness to doxycycline in mice and Non-Human primates
Lamartina, S., Silvi, L., Roscilli, G., Casimiro, D., Simon, A. J., Davies, M., Shiver, J., Rinaudo, C. D., Zampaglione, I., Fattori, E., et al. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(02)00051-5 · Cited by 75
The Four Distal Tyrosines Are Required for LAT-dependent Signaling in FcεRI-mediated Mast Cell Activation
Saitoh, S.-I., Odom, S., Gomez, G., Sommers, C. L., Young, H. A., Rivera, J., & Samelson, L. E. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20030574 · Cited by 84
Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI
Chinnasamy, D., Milsom, M. D., Shaffer, J., Neuenfeldt, J., Shaaban, A. F., Margison, G. P., Fairbairn, L., & Chinnasamy, N. (2006) · Virology Journal · doi:10.1186/1743-422x-3-14 · Cited by 81
Efficient Multicistronic Expression of a Transgene in Human Embryonic Stem Cells
Hasegawa, K., Cowan, A. B., Nakatsuji, N., & Suemori, H. (2007) · Stem Cells · doi:10.1634/stemcells.2006-0813 · Cited by 81
Whirlin Replacement Restores the Formation of the USH2 Protein Complex in Whirlin Knockout Photoreceptors
Zou, J., Luo, L., Shen, Z., Chiodo, V. A., Ambati, B. K., Hauswirth, W. W., & Yang, J. (2011) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.10-6141 · Cited by 80
Efficient control of gene expression in the hematopoietic system using a single Tet-on inducible lentiviral vector
Barde, I., Zanta, M. A., Paisant, S., Leboeuf, M., Rameau, P., Delenda, C., & Danos, O. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2005.09.012 · Cited by 82
Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter
Markusic, D. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni062 · Cited by 78
Use of the 2A Peptide for Generation of Multi-Transgenic Pigs through a Single Round of Nuclear Transfer
Deng, W. W., Yang, D., Zhao, B., Ouyang, Z., Song, J. W., Fan, N., Liu, Z., Zhao, Y., Wu, Q., Nashun, B., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019986 · Cited by 77
Prostate-specific expression of Bax delivered by an adenoviral vector induces apoptosis in LNCaP prostate cancer cells
Lowe, S., Rubinchik, S., Honda, T., McDonnell, T. J., Dong, J.-Y., & Norris, J. (2001) · Gene Therapy · doi:10.1038/sj.gt.3301531 · Cited by 78
CACNA1HM1549V Mutant Calcium Channel Causes Autonomous Aldosterone Production in HAC15 Cells and Is Inhibited by Mibefradil
Reimer, E. N., Walenda, G., Seidel, E., & Scholl, U. I. (2016) · Endocrinology · doi:10.1210/en.2016-1170 · Cited by 76
The impact of telomere erosion on memory CD8+ T cells in patients with X-linked lymphoproliferative syndrome
Plunkett, F. J., Franzese, O., Belaramani, L. L., Fletcher, J. M., Gilmour, K. C., Sharifi, R., Khan, N., Hislop, A. D., Cara, A., Salmon, M., et al. (2005) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2005.03.006 · Cited by 74
Memory in aged mice is rescued by enhanced expression of the GluN2B subunit of the NMDA receptor
Brim, B. C., Haskell, R. E., Awedikian, R., Ellinwood, N. M., Jin, L. E., Kumar, A., Foster, T. C., & Magnusson, K. R. (2012) · Behavioural Brain Research · doi:10.1016/j.bbr.2012.10.026 · Cited by 73
Simian Immunodeficiency Virus (SIV)-Specific Chimeric Antigen Receptor-T Cells Engineered to Target B Cell Follicles and Suppress SIV Replication
Haran, K. P., Hajduczki, A., Pampusch, M. S., Mwakalundwa, G., Vargas‐Inchaustegui, D. A., Rakasz, E. G., Connick, E., Berger, E. A., & Skinner, P. J. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00492 · Cited by 73
Three CNGC Family Members, CNGC5, CNGC6, and CNGC9, Are Required for Constitutive Growth of Arabidopsis Root Hairs as Ca2+-Permeable Channels
Tan, Y.-Q., Yang, Y., Zhang, A., Fei, C.-F., Gu, L.-L., Sun, S., Xu, W. M., Wang, L., Liu, H., & Wang, Y. (2019) · Plant Communications · doi:10.1016/j.xplc.2019.100001 · Cited by 70
Bidirectional promoter interference between two widely used internal heterologous promoters in a late-generation lentiviral construct
Curtin, J. A., Dane, A. P., Swanson, A., Alexander, I. E., & Ginn, S. L. (2008) · Gene Therapy · doi:10.1038/sj.gt.3303105 · Cited by 70
Tobacco Etch Virus protease: A shortcut across biotechnologies
Cesaratto, F., Burrone, Ó. R., & Petris, G. (2016) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2016.06.012 · Cited by 70
Use of HIV as a gene transfer vector.
Pluta, K. D., & Kacprzak, M. M. (2009) · Acta Biochimica Polonica · doi:10.18388/abp.2009_2490 · Cited by 76
Plasticity of Intact Rubral Projections Mediates Spontaneous Recovery of Function after Corticospinal Tract Injury
Siegel, C. S., Fink, K. L., Strittmatter, S. M., & Cafferty, W. B. J. (2015) · Journal of Neuroscience · doi:10.1523/jneurosci.3713-14.2015 · Cited by 72
Primary Polyclonal Human T Lymphocytes Targeted to Carcino-Embryonic Antigens and Neural Cell Adhesion Molecule Tumor Antigens by CD3ζ-Based Chimeric Immune Receptors
Gilham, D. E., O'Neil, A., Hughes, C., Guest, R. D., Kirillova, N., Lehane, M., & Hawkins, R. E. (2002) · Journal of Immunotherapy · doi:10.1097/00002371-200203000-00002 · Cited by 67
Cellular Reprogramming of Human Peripheral Blood Cells
Zhang, X. (2013) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2013.09.001 · Cited by 66
Retrovirus and lentivirus vector design and methods of cell conditioning
Cooray, S., Howe, S. J., & Thrasher, A. J. (2012) · Methods in Enzymology · doi:10.1016/b978-0-12-386509-0.00003-x · Cited by 65
Acute suppression of LH secretion by prolactin in female mice is mediated by kisspeptin neurons in the arcuate nucleus
Brown, R. S. E., Khant Aung, Z., Phillipps, H. R., Barad, Z., Lein, H.-J., Boehm, U., Szawka, R. E., & Grattan, D. R. (2019) · Endocrinology · doi:10.1210/en.2019-00038 · Cited by 62
Neurodevelopmental Role for VGLUT2 in Pyramidal Neuron Plasticity, Dendritic Refinement, and in Spatial Learning
He, H., Mahnke, A. H., Doyle, S., Fan, N., Wang, C.-C., Hall, B. J., Tang, Y., Inglis, F. M., Chen, C., & Erickson, J. D. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.4505-11.2012 · Cited by 67
Optimal promoter usage for lentiviral vector-mediated transduction of cultured central nervous system cells
Li, M., Husic, N., Lin, Y., Christensen, H. M., Malik, I., McIver, S. R., Daniels, C. M. L., Harris, D. A., Kotzbauer, P. T., Goldberg, M. P., et al. (2010) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2010.03.019 · Cited by 61
The Human Cytomegalovirus Immediate‐Early Promoter is Transcriptionally Active in Undifferentiated Mouse Embryonic Stem Cells
Ward, C. M., & Stern, P. L. (2002) · Stem Cells · doi:10.1634/stemcells.20-5-472 · Cited by 61
Use of Replication-Conditional Adenovirus as a Helper System to Enhance Delivery of P450 Prodrug-Activation Genes for Cancer Therapy
Jounaïdi, Y., & Waxman, D. J. (2004) · Cancer Research · doi:10.1158/0008-5472.can-03-1798 · Cited by 61
Quantitative evaluation of MRI-based tracking of ferritin-labeled endogenous neural stem cell progeny in rodent brain
Velde, G. V., Rangarajan, J. R., Vreys, R., Guglielmetti, C., Dresselaers, T., Verhoye, M., der Linden, A. V., Debyser, Z., Baekelandt, V., Maes, F., et al. (2012) · NeuroImage · doi:10.1016/j.neuroimage.2012.04.040 · Cited by 60
Production of protein complexes via co-expression
Kerrigan, J. J., Xie, Q. S., Ames, R. S., & Lu, Q. (2010) · Protein Expression and Purification · doi:10.1016/j.pep.2010.07.015 · Cited by 61
The Gal4/UAS toolbox in zebrafish: new approaches for defining behavioral circuits
Scott, E. K. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.06161.x · Cited by 64
Vectorology and Factor Delivery in Induced Pluripotent Stem Cell Reprogramming
Hu, K. (2014) · Stem Cells and Development · doi:10.1089/scd.2013.0621 · Cited by 61
Improvement of reporter activity by IRES-mediated polycistronic reporter system
Bouabe, H., Fässler, R., & Heesemann, J. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkm1119 · Cited by 60
Human Embryonic Stem Cells and Gene Therapy
Strulovici‐Barel, Y., Leopold, P. L., O’Connor, T. P., Pergolizzi, R. G., & Crystal, R. G. (2007) · Molecular Therapy · doi:10.1038/mt.sj.6300125 · Cited by 59
High-resolution mouse subventricular zone stem-cell niche transcriptome reveals features of lineage, anatomy, and aging
Xie, X., Laks, D. R., Sun, D., Poran, A., Laughney, A. M., Wang, Z., Sam, J., Belenguer, G., Fariñas, I., Elemento, O., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2014389117 · Cited by 66
Bundling mRNA Strands to Prepare Nano‐Assemblies with Enhanced Stability Towards RNase for In Vivo Delivery
Yoshinaga, N., Cho, E., Koji, K., Mochida, Y., Naito, M., Osada, K., Kataoka, K., Cabral, H., & Uchida, S. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201905203 · Cited by 57
A novel ‘sort-suicide’ fusion gene vector for T cell manipulation
Fehse, B., Kustikova, O. S., Li, Z., Wahlers, A., Bohn, W., Beyer, W. R., Chalmers, D., Tiberghien, P., Kühlcke, K., Zander, A. R., et al. (2002) · Gene Therapy · doi:10.1038/sj.gt.3301828 · Cited by 56
Lymph node macrophages drive immune tolerance and resistance to cancer therapy by induction of the immune-regulatory cytokine IL-33
Lamorte, S., Quevedo, R., Jin, R., Neufeld, L. A., Liu, Z. Q., Ciudad, M. T., Lukhele, S., Bruce, J. K., Mishra, S. V., Zhang, X., et al. (2025) · Cancer Cell · doi:10.1016/j.ccell.2025.02.017 · Cited by 55
Comparison of red-shifted firefly luciferase Ppy RE9 and conventional Luc2 as bioluminescence imaging reporter genes for in vivo imaging of stem cells
Liang, Y., Walczak, P., & Bulte, J. W. (2012) · Journal of Biomedical Optics · doi:10.1117/1.jbo.17.1.016004 · Cited by 57
Multigene Lentiviral Vectors Based on Differential Splicing and Translational Control
Zhu, Y., Feuer, G., Day, S. L., Wrzesinski, S. H., & Planelles, V. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0469 · Cited by 54
Tight Positive Regulation of Transgene Expression by a Single Adenovirus Vector Containing the rtTA and tTS Expression Cassettes in Separate Genome Regions
Mizuguchi, H., Xu, Z.-L., Sakurai, F., Mayumi, T., & Hayakawa, T. (2003) · Human Gene Therapy · doi:10.1089/104303403767740803 · Cited by 61
Self-citation
The hydrodynamics-based procedure for controlling the pharmacokinetics of gene medicines at whole body, organ and cellular levels.
Kobayashi, N., Nishikawa, M., & Takakura, Y. (2005) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2004.12.006 · Cited by 62
Preclinical Assessment of Suitable Natural Killer Cell Sources for Chimeric Antigen Receptor Natural Killer–Based “Off-the-Shelf” Acute Myeloid Leukemia Immunotherapies
Kloeß, S., Oberschmidt, O., Dahlke, J., Vu, X., Neudoerfl, C., Kloos, A., Gardlowski, T., Matthies, N., Heuser, M., Meyer, J., et al. (2019) · Human Gene Therapy · doi:10.1089/hum.2018.247 · Cited by 53
In vitro and in vivo comparison of viral and cellular internal ribosome entry sites for bicistronic vector expression
Licursi, M., Christian, S. L., Pongnopparat, T., & Hirasawa, K. (2011) · Gene Therapy · doi:10.1038/gt.2011.11 · Cited by 52
Novel Highly Sensitive IL-10–β-Lactamase Reporter Mouse Reveals Cells of the Innate Immune System as a Substantial Source of IL-10 In Vivo
Bouabe, H., Liu, Y., Moser, M., Bösl, M. R., & Heesemann, J. (2011) · The Journal of Immunology · doi:10.4049/jimmunol.1101477 · Cited by 54
A High-Throughput Platform for Lentiviral Overexpression Screening of the Human ORFeome
Škalamera, D., Ranall, M. V., Wilson, B. M., Leo, P. J., Purdon, A. S., Hyde, C., Nourbakhsh, E., Grimmond, S. M., Barry, S. C., Gabrielli, B. G., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0020057 · Cited by 50
Production of chimeric monoclonal antibodies by genetically manipulated chickens
Kamihira, M., Kawabe, Y., Shindo, T., Ono, K.-I., Esaka, K., Yamashita, T., Nishijima, K.-I., & Iijima, S. (2009) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2009.02.022 · Cited by 51
In vivo imaging of colorectal cancer growth and metastasis by targeting MACC1 with shRNA in xenografted mice
Pichorner, A., Sack, U., Kobelt, D., Kelch, I. D., Arlt, F., Smith, J. L., Walther, W., Schlag, P. M., & Stein, U. (2012) · Clinical & Experimental Metastasis · doi:10.1007/s10585-012-9472-6 · Cited by 49
TRPC channels regulate Ca2+-signaling and short-term plasticity of fast glutamatergic synapses
Schwarz, Y. N., Oleinikov, K., Schindeldecker, B., Wyatt, A. K., Weißgerber, P., Flockerzi, V., Boehm, U., Freichel, M., & Bruns, D. (2019) · PLoS Biology · doi:10.1371/journal.pbio.3000445 · Cited by 48
Retroviral Vectors for Gene Therapy
Maier, P., Kalle, C. V., & Laufs, S. (2010) · Future Microbiology · doi:10.2217/fmb.10.100 · Cited by 51
2A to the Fore – Research, Technology and Applications
Luke, G. A., Escuin-Ordinas, H., de Felipe, P., & Ryan, M. D. (2009) · Biotechnology and Genetic Engineering Reviews · doi:10.5661/bger-26-223 · Cited by 48
Lentiviral transduction of human hematopoietic cells by HIV‐1‐ and SIV‐based vectors containing a bicistronic cassette driven by various internal promoters
Dupuy, F. P., Mouly, E., Mesel‐Lemoine, M., Morel, C., Abriol, J., Cheraï, M., Baillou, C., Négre, D., Cosset, F., Klatzmann, D., et al. (2005) · The Journal of Gene Medicine · doi:10.1002/jgm.769 · Cited by 52

Authors: Mizuguchi, H., Xu, Z., Ishii-Watabe, A., Uchida, E., & Hayakawa, T.
Affiliations: National Institute of Health Sciences (JP)
Corresponding author: Mizuguchi, Hiroyuki
ORCID: Mizuguchi, Hiroyuki
Xu, Zhili
Hayakawa, Takao
Venue: Molecular Therapy
Publisher: Elsevier BV
Published: 2000-04
Volume: 1
Issue: 4
Pages: 376-382
ISSN: 1525-0016 (ISSN-L)
DOI: 10.1006/mthe.2000.0050
OpenAlex: W2000853857
Cited by: 559
References: 34
Citations by year: 2026: 12 · 2025: 25 · 2024: 24 · 2023: 37 · 2022: 17 · 2021: 20 · 2020: 23 · 2019: 26 · 2018: 24 · 2017: 15 · 2016: 19 · 2015: 21 · 2014: 24 · 2013: 24 · 2012: 35
FWCI: 4.34
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-nd
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by-nc-nd/4.0/ (vor, 6033 d)
Locations: Molecular Therapy (publishedVersion, cc-by-nc-nd)
PubMed (publishedVersion)
Funders: National Institutes of Health
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords: internal ribosome entry site, bicistronic vector, cap-dependent translation, encephalomyocarditis virus, 5' untranslated region, gene expression efficiency
MeSH terms: Alkaline Phosphatase, Animals, Encephalomyocarditis virus, Genes, Genetic Vectors, Cricetinae, HeLa Cells, Humans, L Cells, Liver, Luciferases, Plasmids, RNA Caps, Chloramphenicol O-Acetyltransferase, Gene Expression, CHO Cells, Gene Transfer Techniques, 5' Untranslated Regions, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

IRES-Dependent Second Gene Expression Is Significantly Lower Than Cap-Dependent First Gene Expression in a Bicistronic Vector

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Citations

Cites

Cited by

Related

Metadata

Record

About these checks