The Complete Nucleotide Sequence of Parvovirus LuIII and Localization of a Unique Sequence Possibly Responsible for Its Encapsidation Pattern
Abstract
Parvovirus LuIII encapsidates single-stranded DNA of either plus or minus polarity with equal frequency, whereas the rodent parvoviruses MVMp and H-1 encapsidate minus strand DNA only. A full-length, infectious clone of LuIII was constructed and the complete nucleotide sequence of the genome was determined. Comparison of the LuIII sequence with those of MVMp and H-1 revealed that these viruses are virtually identical with respect to the genomic organization, location of regulatory signals, mRNA splicing patterns, and amino acid sequences of viral proteins. However, two regions of the LuIII sequence differ significantly from those of the rodent parvoviruses. At mu 92, LuIII has only one copy of a sequence found as a direct repeat in MVMp and H-1. Upstream of this sequence, at mu 89, there is an A-T-rich region, 47 nucleotides in length, unique to the LuIII genome. This A-T-rich region could represent a signal responsible for the totally different encapsidation patterns observed for these viruses.
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The maximum packaging capacity of members of the Parvoviridae family is approximately 4.5-5.5 kb.
“This suggests that the maximum packaging capacity of members of the Parvoviridae family is approximately 4.5-5.5 kb.”
From Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids (2016) · cites this paper as [23]
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Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids
Chamberlain, K., Riyad, J. M., & Weber, T. (2016) · Human Gene Therapy Methods · doi:10.1089/hgtb.2015.140 · Cited by 141 · see citing works
- Authors
- Diffoot, N., Chen, K. C., Bates, R. C., & Lederman, M.
- Affiliations
- Virginia Tech (US)
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 1993-01
- Volume
- 192
- Issue
- 1
- Pages
- 339-345
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W2038021574
- Cited by
- 23
- References
- 0
- Citations by year
- 2022: 1 · 2017: 1 · 2016: 1 · 2013: 1
- FWCI
- 3.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Virology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parvovirus B19 Infection Studies
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- viral encapsidation, A+T-rich region, direct repeats, parvovirus H-1, viral genome structure, mRNA splicing, infectious clone
- MeSH terms
- Base Sequence, Capsid, Cloning, Molecular, DNA, Viral, Genes, Viral, Molecular Sequence Data, Parvoviridae, Viral Structural Proteins, Open Reading Frames, Sequence Alignment, Viral Nonstructural Proteins, Sequence Homology, Amino Acid
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