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Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells
Abstract
Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.
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“when NG2+ cells were removed from m-BMMSCs by magnetic activated cell sorting [MACS, m-NG2(-)/BMMSCs] [37], no improvements in biliary repair were detected; these effects were represented by CK7 (Fig. 4Ea, boxes, b, p = 0.931) or CK19 (Fig. 4Fa, boxes, b, p = 0.386) expression, comparable to DEN mice”
From New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis (2024) · cites this paper as [37]
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Research works printed but not linked: 4
- Grutzkau A, Radbruch A (2010) Small but mighty: how the MACS-technology based on nanosized superparamagnetic particles has helped to analyze the immune system within the last 20 years. Cytometry A 77(7):643–647. https://doi.org/10.1002/cyto.a.20918 · DOI
- Abts H, Emmerich M, Miltenyi S, Radbruch A, Tesch H (1989) CD20 positive human B lymphocytes separated with the magnetic cell sorter (MACS) can be induced to proliferation and antibody secretion in vitro. J Immunol Methods 125(1–2):19–28 · DOI
- Will B, Steidl U (2010) Multi-parameter fluorescence-activated cell sorting and analysis of stem and progenitor cells in myeloid malignancies. Best Pract Res Clin Haematol 23(3):391–401. https://doi.org/10.1016/j.beha.2010.06.006 · DOI
- Kose S, Aerts-Kaya F, Kopru CZ, Nemutlu E, Kuskonmaz B, Karaosmanoglu B, Taskiran EZ, Altun B, Uckan Cetinkaya D, Korkusuz P (2018) Human bone marrow mesenchymal stem cells secrete endocannabinoids that stimulate in vitro hematopoietic stem cell migration effectively comparable to beta-adrenergic stimulation. Exp Hematol 57:30–41.e31. https://doi.org/10.1016/j.exphem.2017.09.009 · DOI
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Hu, D., Lai, J., Chen, Q., & Bai, L. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03817-x · Cited by 6
“…endogenous cholangiocyte-mediated hepatocyte regeneration in the diseased liver. owever, when NG2 + cells were removed from m-BM MSCs by magnetic activated cell sorting [MACS, m-NG2(-)/ BM MSCs] [37], no improvements in biliary repair were detected; these effects were represented by CK7 (Fig.”
Background
Metadata
- Authors
- Korkusuz, P., Köse, S., Yersal, N., & Önen, S.
- Affiliations
- Hacettepe University (TR)
- Atilim University (TR)
- Corresponding author
- Korkusuz, Petek
- Venue
- Methods in Molecular Biology
- Publisher
- Springer New York
- Published
- 2018
- Volume
- 1879
- Pages
- 153-163
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2897698671
- Cited by
- 16
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 2
- FWCI
- 3.79
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- magnetic cell separation, stem cell isolation, skin stem cells, hematopoietic stem cells, spermatogonial stem cells, CD34, CD90, cell purification
- MeSH terms
- Animals, Cell Separation, Hematopoietic Stem Cells, Humans, Magnetics, Mice, Inbred C57BL, Stem Cells, Immunomagnetic Separation, Hematopoietic Stem Cell Transplantation, Thy-1 Antigens, Antigens, CD34, Mice
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