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Plasmid-Based Reverse Genetics of Influenza A Virus
Abstract
Reverse genetics is the process of generating an RNA virus from a cDNA copy. Reverse genetics systems have truly transformed our ability to manipulate and study negative-strand RNA viruses. Plasmid-based reverse genetics approaches for influenza viruses provide a better understanding of virulence, transmission, mechanisms of antiviral resistance, and the development of alternative vaccines and vaccination strategies. Studying the molecular changes that allow influenza A viruses (IAVs) to transmit among animal species is important to better understand their animal health and public health risks. In this chapter, the cloning of cDNA copies of IAV's RNA segments into a reverse genetics plasmid vector, the experimental procedures for studying viral polymerase activity, and the successful generation of recombinant IAVs are described.
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From Human innovation and the creative agency of the world in the age of generative AI (2024) · cites this paper as [11]
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Research works printed but not linked: 15
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- Imai M, Watanabe T, Hatta M, Das SC, Ozawa M, Shinya K, Zhong G, Hanson A, Katsura H, Watanabe S, Li C, Kawakami E, Yamada S, Kiso M, Suzuki Y, Maher EA, Neumann G, Kawaoka Y (2012) Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets. Nature 486(7403):420–428. https://doi.org/10.1038/nature10831 · DOI · Paper page
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Cited by
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Human innovation and the creative agency of the world in the age of generative AI
Peschl, M. F. (2024) · Possibility Studies & Society · doi:10.1177/27538699241238049 · Cited by 30
Metadata
- Authors
- Perez, D. R., Seibert, B., Ferreri, L., Lee, C.-W., & Rajao, D.
- Affiliations
- University of Georgia (US)
- The Ohio State University (US)
- Corresponding author
- Perez, Daniel R.
- Venue
- Methods in Molecular Biology
- Publisher
- Springer US
- Published
- 2020
- Pages
- 37-59
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W4243682365
- Cited by
- 19
- References
- 0
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2022: 4 · 2021: 2 · 2020: 2
- FWCI
- 8.52
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in molecular biology (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (HHSN272201400008C), R21AI146448)
- Topics
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- influenza A virus, cDNA cloning, negative-strand RNA viruses, recombinant virus generation, viral polymerase activity, antiviral resistance, animal-to-human transmission, influenza vaccination, viral virulence
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- CoChat. (2026). Citation check: Plasmid-Based Reverse Genetics of Influenza A Virus. https://app.cochat.ai/papers/10.1007/978-1-0716-0346-8_4
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- Perez, D. R., Seibert, B., Ferreri, L., Lee, C.-W., & Rajao, D. (2020). Plasmid-Based Reverse Genetics of Influenza A Virus. Methods in Molecular Biology. https://doi.org/10.1007/978-1-0716-0346-8_4
