Pre-embedding immunostaining of brain tissue and three-dimensional imaging with FIB-SEM
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In a prior study, asymmetric and symmetric synapses were clearly identified and distinguished from one another in material prepared without potassium ferrocyanide.
“This material was prepared without potassium ferrocyanide, and the AS and SS were clearly identified and distinguished from one another (Turégano-López et al., 2021).”
From Unambiguous identification of asymmetric and symmetric synapses using volume electron microscopy (2024) · cites this paper as Turégano-López et al., 2021
The morphology of the synaptic junctions formed by VGAT-positive boutons in material not treated with potassium ferrocyanide is similar to that observed in brain sections treated with 0.1% potassium ferrocyanide.
“As depicted in Figure 7, the morphology of the synaptic junctions formed by VGAT-positive boutons in this material (without potassium ferrocyanide) is similar to those observed in brain sections using 0.1% potassium ferrocyanide (Figure 4).”
From Unambiguous identification of asymmetric and symmetric synapses using volume electron microscopy (2024) · cites this paper as Turégano-López et al. (2021)
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References 34 · Cited by 5
- Authors
- Turégano-López, M., Rodríguez, J.-R., Alonso-Nanclares, L., González-Soriano, J., DeFelipe, J., & Merchán-Pérez, A.
- Affiliations
- Universidad Politécnica de Madrid (ES)
- Consejo Superior de Investigaciones Científicas (ES)
- Venue
- Neuromethods
- Publisher
- Springer US
- Published
- 2021
- Pages
- 285-302
- ISSN
- 0893-2336 (ISSN-L)
- 1940-6045
- OpenAlex
- W3198529630
- Cited by
- 5
- References
- 34
- Citations by year
- 2026: 1 · 2024: 2 · 2022: 1 · 2021: 1
- FWCI
- 1.79
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Neuromethods (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Advanced Materials Characterization Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- FIB-SEM, brain tissue, vibratome sections, serial sectioning, 3D imaging, immunocytochemistry, epoxy resin embedding
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