Establishing a New Research Axolotl Colony
Open accessAbstract
The field of regenerative biology has taken a keen interest in the Mexican axolotl (Ambystoma mexicanum) over the past few decades, as this salamander successfully regenerates amputated limbs and injured body parts. Recent progress in research tool development has also made possible axolotl genetic manipulation and single-cell analysis, which will help understand the molecular mechanisms of complex tissue regeneration. To support the growing popularity of this model, we describe how to set up a new axolotl housing facility at a research laboratory. We also review husbandry practices for raising axolotls and using them in biological research, with a focus on diet, water quality, breeding, and anesthesia.
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Tiger colour morphs in zoos raise conservation and welfare concerns
Erzinçlioğlu, T. S., Piaz, M. T., Chinpilas, T., Hunnestad, A. V., Godfrey, L., & Fourage, A. (2026) · Discover Animals · doi:10.1007/s44338-026-00163-5
- Authors
- Yandulskaya, A. S., & Monaghan, J. R.
- Affiliations
- Northeastern University (US)
- Corresponding author
- Monaghan, James R.
- ORCID
- Monaghan, James R.
- Venue
- Methods in Molecular Biology
- Publisher
- Springer US
- Published
- 2022-10-23
- Volume
- 2562
- Pages
- 27-39
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- PMC
- PMC10948202
- OpenAlex
- W4307159527
- Cited by
- 5
- References
- 0
- Citations by year
- 2026: 2 · 2025: 3
- FWCI
- 1.26
- Type
- book chapter
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (P40OD019794)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD099174)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
- Physical Sciences › Environmental Science › Global and Planetary Change › Amphibian and Reptile Biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- axolotl, laboratory colony establishment, animal husbandry, water quality, anesthesia, limb regeneration, regenerative biology
- MeSH terms
- Ambystoma mexicanum, Animals
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