Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells
Abstract
The integrity of the blood-brain barrier (BBB), the protective barrier of the brain, is key to maintaining normal microvascular permeability and brain homeostasis. Brain microvascular endothelial cells are primary components of the blood-brain barrier. Transendothelial electrical resistance (TEER) is the electrical resistance across a cellular monolayer such as the brain microvascular endothelial cell monolayers. Measurement of TEER is considered a sensitive, reliable, and noninvasive method for evaluating barrier integrity and permeability of an endothelial cell monolayer under in vitro conditions. Measurement of TEER is also helpful for studying various cellular and molecular changes and signaling events that regulate barrier functions in endothelial monolayers. One of the in vitro endothelial cell barrier models that have been commonly used for measuring TEER is the BBB model using human or rodent brain microvascular endothelial cells grown as a monolayer. In this protocol, we describe how TEER is measured in brain microvascular endothelial cell monolayers, to determine blood-brain barrier integrity under in vitro conditions.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Endothelial cells are highly sealed by tight junctions that impede paracellular diffusion of most molecules and cause high transendothelial electrical resistance, restraining passage of substances into the brain.
“ECs are highly sealed by TJs that impede the paracellular diffusion of most molecules, besides causing blood vessels to have high transendothelial electrical resistance, which translates to the integrity of the cellular barriers in restraining the passage of substances into the brain [6,10].”
From Advances in Strategies to Transport Nanoparticles Across the Blood–Brain Barrier for Drug Delivery into the Brain for the Treatment of Alzheimer’s Disease (2026) · cites this paper as [10]
The citation lists haven’t been fetched yet
References 11 · Cited by 10
- Authors
- Waithe, O. Y., Peng, X., Childs, E. W., & Tharakan, B.
- Affiliations
- Morehouse School of Medicine (US)
- Corresponding author
- Tharakan, Binu
- ORCID
- Tharakan, Binu
- Venue
- Methods in Molecular Biology
- Publisher
- Springer US
- Published
- 2023-10-01
- Volume
- 2711
- Pages
- 199-203
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W4387218744
- Cited by
- 10
- References
- 11
- Citations by year
- 2026: 7 · 2025: 3
- FWCI
- 9.23
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (SC3NS127765)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- transendothelial electrical resistance, blood-brain barrier, brain microvascular endothelial cells, endothelial monolayer, barrier integrity, microvascular permeability, in vitro BBB model, endothelial barrier function
- MeSH terms
- Blood-Brain Barrier, Brain, Capillary Permeability, Cells, Cultured, Humans, Electric Impedance, Endothelial Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
