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The Enzymatic Coagulation of Milk
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“Rennet-altered CN micelles are susceptible to aggregation due to the reduction in steric and charge repulsion (between particles) caused by the loss of the macropeptide from κ-CN.”
From Invited review: perspectives on the basis of the rheology and texture properties of cheese. (2003) · cites this paper as Dalgleish, 1993
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Basset, M., Monteil, J., Demaille, V., Faure, B., Leal-Calderón, F., Harscoat-Schiavo, C., & Savoire, R. (2023) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2023.111651 · Cited by 1
An Efficient Ultrasonic Method to Control the Coagulation Enzymatic of Milk
Bakkali, F., Derra, M., Amghar, A., & Sahsah, H. (2017) · doi:10.22266/ijies2017.0831.20 · Cited by 1
“The lack of a rigorous cutting time characterization and the usual changes in milk protein content are forcing modern cheese plants to find other methods in order to control coagulation, gel firming, curd syneresis, cheese yield, and product quality [2-4].”
BackgroundPRESERVATION OF RAW COW MILK USING NON THERMAL TREATMENTS
El-Dieb, S., Sobhy, H., Emara, E., el-Nawawy, M., & El-Aidie, S. A. M. (2014) · doi:10.21608/jfds.2014.52743 · Cited by 1
“The effect of heat treatment on coagulation time was agreement with (Dalgleish, 1992; Lucey, 1995; Hyslop, 2003; Anema et al. 2007; Blecker et al. 2012), they found that heat treatments of milk at relatively high”
BackgroundInvestigations into Structure Formation of Dairy Products
Brückner-Gühmann, M. (2011) · doi:10.14279/depositonce-3045 · Cited by 1
“The release of GMP is completed after 25 min whereas in the case of goat milk this lasts up to 50 min. Goat milk was found to contain more -casein [15;43] than cow milk. Nevertheless, RICHARDSON, CREAMER, and MUNFORD (1973) reported that the composition of caprine -casein was very similar to that of bovine milk in…”
BackgroundMethodResultInfluentialPrediction of clotting time for milk coagulation by mixtures of proteolytic enzymes
Picon, A., Medina, M., Gaya, P., & Núñez, M. (1994) · doi:10.1016/0308-8146(95)93291-x · Cited by 1
Ultrasonic measurement of milk coagulation time
Bakkali, F., Moudden, A., Faiz, B., Amghar, A., Maze, G., de Espinosa, F. D., & Akhnak, M. (2001) · doi:10.1088/0957-0233/12/12/317
Unmasking the internal structure of casein micelles through enzymatic hydrolysis: A SAXS study
Bauland, J., Messaoud, G. B., Boué, F., Hamon, P., Rousseau, F., Gibaud, T., & Croguennec, T. (2026) · doi:10.48550/arxiv.2608.19969
“Enzymatic milk gelation is a multi-step process involving (i) enzymatic hydrolysis of κ -casein, (ii) aggregation of destabilized casein micelles, and (iii) gel formation and aging [8, 9].”
Hamouda, M. E. A., Don, P. U., & Salunke, P. (2026) · Journal of Dairy Science · doi:10.3168/jds.2026-28822
The effect of curd cutting time and coagulants on the efficiency of cheesemaking
Stocco, G., Casali, D., Valoppi, D., Tedeschi, I., Concar, E., Lantz, S., Goodwins, J., & Cipolat‐Gotet, C. (2026) · Journal of Dairy Science · doi:10.3168/jds.2026-28341
Sibono, L., Grosso, M., Errico, M., Tronci, S., Lai, G., Pes, M., Addis, M., Caboni, P., & Manis, C. (2025) · Food Research International · doi:10.1016/j.foodres.2025.117987
Akgün, H., & Taş, T. K. (2025) · Small Ruminant Research · doi:10.1016/j.smallrumres.2025.107648
Utilization of Natural Milk Coagulant in The Processing of Indigenous Dairy Product (Palopo)
Agil, A., Bulkaini, B., Wulandani, B. R. D., Kisworo, D., & Fahrullah, F. (2025) · JURNAL BIOLOGI TROPIS · doi:10.29303/jbt.v25i4.10356
“Proses koagulasi terjadi melalui pemutusan ikatan peptida spesifik pada κ -kasein sehingga micelle kasein menjadi tidak stabil dan mengendap, tanpa menghasilkan senyawa asam dalam jumlah berarti (Fox et al ., 2017).”
Belen'kaya, S. V., G., L. M., Borisevich, S. S., Khamitov, E., Diusenova, S., Shevtsov, M. B., Borshchevskiy, V. I., Kolosov, P. V., Fomin, A., Arkhipov, S. Г., et al. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.148085
Ren, J., Qian, Y., Liao, M., Li, K., Ma, L.-J., Chen, F., Hu, X.-S., & Ji, J.-F. (2024) · Colloids and Surfaces A: Physicochemical and Engineering Aspects · doi:10.1016/j.colsurfa.2024.134847
Ibrahim, A., & Abdeen, E. (2022) · Journal of Food and Dairy Sciences · doi:10.21608/jfds.2022.174356.1079
“Fox et al., (2017) observed a similar trend and reported that the flocculation time was inversely proportional to the enzyme concentration.”
BackgroundPreliminary Study on the Role of Ionic Calcium in Gelation and Proteolysis of UHT Milk
Mlipano, C. L., & Michael, L. (2017) · doi:10.5296/jfs.v7i1.12081
“It is essential for gelation of milk by rennet during cheese manufacture (Tsioulpas, 2005; Hyslop, 2003).”
BackgroundLazzaro, F. (2017) · doi:10.70675/652a002ez73fbz41c7zb063zba7b43126d7b
“Lowering the pH caused also the release of all type of caseins in ultracentrifuge supernatants as mentioned by Dalgleish & Law (1988), Gastaldi et al.”
BackgroundInfluentialRomeih, E., & Skeie, S. (2013) · doi:10.21608/jfds.2013.71861
“The unfolded whey proteins then interact with k-casein which in turn retards the primary phase (lag phase) of rennet coagulation (Dalgleish, 1992; Singh and Waungana, 2001).”
BackgroundPlace de la matière azotée dans le mécanisme de la coagulation présure du lait
Ilboudo, A., Savadogo, A., Mg, S., & Traore, A. S. (2013) · International Journal of Biological and Chemical Sciences · doi:10.4314/ijbcs.v6i6.35 · Cited by 1
Cheese: Rennet Coagulation of Milk
Lucey, J. (2021) · Reference Module in Food Science · doi:10.1016/b978-0-12-818766-1.00209-9
Rennet-induced gels and their mechanical properties
Choi, J.-W. (2011) · Korean Journal of Agricultural Science · doi:10.7744/cnujas.2011.38.4.711
Metadata
- Authors
- Dalgleish, D. G.
- Affiliations
- University of Guelph (CA)
- Corresponding author
- Dalgleish, D. G.
- Venue
- Cheese: Chemistry, Physics and Microbiology
- Publisher
- Springer US
- Published
- 1993
- Volume
- 1
- Pages
- 69-100
- OpenAlex
- W1881198270
- Cited by
- 216
- References
- 0
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 2 · 2023: 4 · 2022: 8 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 9 · 2016: 5 · 2015: 6 · 2014: 12 · 2013: 9 · 2012: 3
- FWCI
- 23.60
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Springer eBooks (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Milk Quality and Mastitis in Dairy Cows
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Keywords
- milk coagulation, rennet, chymosin, kappa-casein, casein micelles, proteolytic enzymes, pepsin, cheese production, micelle aggregation
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