Agrobacterium-Mediated Transformation of Barley (Hordeum vulgare L.)
Abstract
Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Other cultivars such as 'Compass', 'Spartacus', and the breeding line WI4330 have been used in previous research, but with low efficiency.
“Other cultivars such as 'Compass', 'Spartacus', and the breeding line WI4330 have been used in previous research, but with low efficiency (Ismagul et al., 2014;Han et al., 2021;Karunarathne et al., 2022).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Ismagul et al., 2014
The citation lists haven’t been fetched yet
References 9 · Cited by 11
- Authors
- Ismagul, A., Mazonka, I., Callegari, C., & Eliby, S.
- Affiliations
- Australian Centre for Plant Functional Genomics (AU)
- Corresponding author
- Eliby, Serik
- ORCID
- Eliby, Serik
- Venue
- Methods in Molecular Biology
- Publisher
- Springer New York
- Published
- 2014
- Volume
- 1145
- Pages
- 203-211
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2395899587
- Cited by
- 11
- References
- 9
- Citations by year
- 2026: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2016: 1 · 2014: 2
- FWCI
- 1.27
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- Agrobacterium-mediated transformation, barley, transgenic plants, reverse genetics
- MeSH terms
- Hordeum, Genetic Engineering, Seeds, Transformation, Genetic, Genes, Plant, Plants, Genetically Modified, Agrobacterium
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
