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Cited by 12Extracellular vesicles in diseaseMicroRNA in disease regulationCancer-related molecular mechanisms research

Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts

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Bhome, Rahul, Goh, Rebecca, Pickard, Karen, Mellone, Massimiliano, Sayan, A. Emre, Mirnezami, Alex·2017·Methods in Molecular Biology
DOI 10.1007/978-1-4939-6524-3_11PMC PMC5757798
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Abstract

The tumor microenvironment is a heterogeneous and dynamic network that exists between cancer and stroma, playing a critical role in cancer progression. Certain tumorigenic signals such as microRNAs are derived from the stroma and conveyed to cancer cells (and vice versa) in nanoparticles called exosomes. Their identification and characterization is an important step in better understanding cellular cross talk and its consequences. To this end we describe how to culture primary ex vivo derived fibroblasts from colorectal tissue, isolate their exosomes, extract exosomal RNA and perform microRNA profiling.

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Crossref · 10.1007/978-1-4939-6524-3_11

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  • CAFs are a substantial source of growth factors, cytokines and exosomes that can promote tumour growth and modulate therapy responses.

    “CAFs are also a substantial source of growth factors, cytokines and exosomes that can promote tumour growth and modulate therapy responses27,106–108.”

    From A framework for advancing our understanding of cancer-associated fibroblasts (2020) · cites this paper as [107]

    The Introduction (page unavailable) says fibroblasts secrete exosomes and links activated stroma to cancer-cell proliferation and chemoresistance. It does not say CAFs are a substantial source of growth factors or cytokines, or establish that their exosomes themselves promote tumour growth. The Notes (page unavailable) also say carcinomatous cells produce three to four times more exosomes than mesenchymal cells, so the claimed scale of CAF exosome production is not established. (LLM, conf=0.86)

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Research works printed but not linked: 16

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Authors
Bhome, R., Goh, R., Pickard, K., Mellone, M., Sayan, A. E., & Mirnezami, A.
Affiliations
  • Southampton General Hospital (GB)
  • University of Southampton (GB)
Corresponding author
Bhome, Rahul
ORCID
Venue
Methods in Molecular Biology
Publisher
Springer New York
Published
2016-11-09
Volume
1509
Pages
115-122
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
DOI
10.1007/978-1-4939-6524-3_11
PMC
PMC5757798
OpenAlex
W2555981673
Cited by
12
References
0
Citations by year
2024: 1 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 1 · 2018: 2 · 2017: 2
FWCI
2.06
Type
book chapter
Language
en
Open access
Licence
www.springer.com/tdm (unspecified)
Locations
  • Methods in molecular biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • ePrints Soton (University of Southampton) (submittedVersion)
Funders
  • Cancer Research UK (A20526, 20526, S_3319)
  • National Institute for Health and Care Research (ACF-2012-26-005)
  • Medical Research Council (MR/R002061/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
Keywords
exosomes, miRNA expression profiling, tumor microenvironment, exosomal RNA, exosome isolation, colorectal cancer
MeSH terms
Cells, Cultured, Colon, Fibroblasts, Humans, Rectum, Biomarkers, Tumor, Colorectal Neoplasms, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, Exosomes, Real-Time Polymerase Chain Reaction, Primary Cell Culture

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