In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase
Abstract
Extracellular vesicles (EVs) are cell-derived vesicles comprising a lipid bilayer and are found in body fluids, such as blood, sweat, and urine. As EVs, especially exosomes, function as endogenous intercellular delivery tools, their roles in various biological events have been extensively investigated. In addition, they are expected to become safe and effective drug delivery systems (DDS) because of their intrinsic nature. In the development of EV-based DDS, as well as in the investigation of the biological functions of EVs, it is important to analyze the in vivo behavior of EVs by tracking them. Therefore, we have developed a sensitive EV-labeling method to track EVs in vivo by designing a fusion protein comprising lactadherin (LA) (alias milk fat globule-EGF factor 8), a protein that binds to EV membranes through interaction with phosphatidylserine, and Gaussia luciferase (gLuc), a chemiluminescent protein. gLuc-LA-labeled EVs are easily obtained by transfecting EV-producing cells with a gLuc-LA-encoding plasmid vector. Here, we describe methods to label EVs with the fusion protein and to track the labeled EVs in vivo.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Phosphatidylserine is present on the outer surface of EVs.
“Since PS is present on the outer surface of EVs40, we next investigated whether the G58 peptide is also responsible for binding of GAPDH to EVs.”
From GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain (2021) · cites this paper as [40]
Cites
Akers, J. C., Gonda, D., Kim, R., Carter, B. S., & Chen, C. C. (2013) · Journal of Neuro-Oncology · doi:10.1007/s11060-013-1084-8 · Cited by 1,423 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Xu, M., Feng, T., Liu, B., Qiu, F., Xu, Y., Zhao, Y., & Zheng, Y. (2021) · Theranostics · doi:10.7150/thno.62330 · Cited by 229 · see citing works
Casella, G., Colombo, F., Finardi, A., Descamps, H., Ill-Raga, G., Spinelli, A., Podini, P., Bastoni, M., Martino, G., Muzio, L., et al. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.06.024 · Cited by 134 · see citing works
Dar, G. H., Mendes, C. C., Kuan, W.-L., Speciale, A. A., Conceição, M., Görgens, A., Uliyakina, I., Lobo, M. J., Lim, W. F., EL Andaloussi, S., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-27056-3 · Cited by 100 · see citing works
- Authors
- Takahashi, Y., Nishikawa, M., & Takakura, Y.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Takahashi, Yuki
- Venue
- Methods in Molecular Biology
- Publisher
- Springer New York
- Published
- 2017
- Volume
- 1660
- Pages
- 245-254
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2746741683
- Cited by
- 34
- References
- 1
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 6 · 2020: 5 · 2019: 4 · 2018: 1
- FWCI
- 3.40
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- extracellular vesicles, exosomes, in vivo tracking, lactadherin, Gaussia luciferase, fusion protein, phosphatidylserine, chemiluminescence, drug delivery systems
- MeSH terms
- Extracellular Vesicles, Animals, Antigens, Surface, Luciferases, Luminescent Measurements, Milk Proteins, Recombinant Fusion Proteins, Transfection, Gene Expression, Genes, Reporter, Mice, Molecular Imaging
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
