CoChat
Sign Up
Cited by 6

The DOI matches its Crossref record.

Correlative Live-Cell Imaging and Super-Resolution Microscopy of Autophagy

Karanasios, Eleftherios·2019·Methods in Molecular Biology
DOI 10.1007/978-1-4939-8873-0_15
Open this paper in CoChat — free account

Abstract

Correlative live-cell imaging and super-resolution microscopy of autophagy was developed to combine the temporal resolution of time-lapse fluorescence microscopy with the spatial resolution of super-resolution microscopy. HEK293 cells that express recombinant proteins of interest fused to fluorescent tags are imaged live to capture the formation of autophagosomes, fixed on stage to "snap-freeze" these structures, stained with appropriate antibodies, relocated, and imaged at super resolution by direct stochastic optical reconstruction microscopy. This chapter provides an easy-to-follow protocol along with practical tips and background information to help set up and perform an experiment.

Overview

Integrity signals

Registry

Crossref · 10.1007/978-1-4939-8873-0_15

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 8

  1. Mizushima N, Komatsu M (2011) Autophagy: renovation of cells and tissues. Cell 147:728–741. https://doi.org/10.1016/j.cell.2011.10.026 · DOI
  2. Karanasios E, Ktistakis NT (2014) Live-cell imaging for the assessment of the dynamics of autophagosome formation: focus on early steps. Methods (San Diego, Calif) 75:54–60. https://doi.org/10.1016/j.ymeth.2014.12.007 · DOI
  3. Karanasios E, Stapleton E, Manifava M et al (2013) Dynamic association of the ULK1 complex with omegasomes during autophagy induction. J Cell Sci 126:5224–5238. https://doi.org/10.1242/jcs.132415 · DOI · Paper page
  4. Koyama-Honda I, Itakura E, Fujiwara TK, Mizushima N (2013) Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9:1491–1499. https://doi.org/10.4161/auto.25529 · DOI
  5. Mizushima N, Klionsky DJ (2007) Protein turnover via autophagy: implications for metabolism. Annu Rev Nutr 27:19–40. https://doi.org/10.1146/annurev.nutr.27.061406.093749 · DOI
  6. Yamamoto H, Kakuta S, Watanabe TM et al (2012) Atg9 vesicles are an important membrane source during early steps of autophagosome formation. J Cell Biol 198:219–233. https://doi.org/10.1083/jcb.201202061 · DOI · Paper page
  7. Walker SA, Karanasios E, Ktistakis NT (2017) Correlative live cell and super resolution imaging of autophagosome formation. Methods Enzymol 587:1–20 · DOI
  8. Theer P, Mongis C, Knop M (2014) PSFj: know your fluorescence microscope. Nat Methods 11:981–982 · DOI

Cited by

Sorted by citations

Metadata

Authors
Karanasios, E.
Venue
Methods in Molecular Biology
Publisher
Springer New York
Published
2019
Pages
231-242
ISSN
  • 1064-3745
  • 1940-6029
DOI
10.1007/978-1-4939-8873-0_15
Cited by
6
References
0
Licence
www.springer.com/tdm (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review