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Cited by 51Neuroscience and Neuropharmacology ResearchNeurogenesis and neuroplasticity mechanismsCell death mechanisms and regulation

Cell Culture of Primary Cerebellar Granule Cells

Krämer, Dana, Minichiello, Liliana·2010·Methods in Molecular Biology
DOI 10.1007/978-1-59745-019-5_17
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Abstract

Cerebellar granule cells are often used as a model system for the study of neuronal development, function and pathology, including the analysis of activity-dependent survival/apoptosis of neurons and the mechanisms of neuroprotection. Cerebellar granule cells are generated postnatally and constitute the largest homogeneous neuronal population of the mammalian brain. In addition, cerebellar granule cells cultured in vitro develop characteristics of mature cerebellar granule cells seen in vivo, such as an extensive neuritic network, expression of excitatory amino acid receptors and production and release of -L: glutamate. Taken together, these features make cerebellar granule cells a unique model system that has been extensively characterised and used for in vitro studies.

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Crossref · 10.1007/978-1-59745-019-5_17

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  • Replacing depolarizing 25 mM KCl-containing medium with 5 mM KCl medium results in rapid apoptotic death of CGN mimicking developmental elimination of cerebellar neurons.

    “Replacing the depolarizing 25 mM KCl-containing medium with 5 mM KCl medium results in a rapid apoptotic death of CGN mimicking the developmental elimination of cerebellar neurons [24,25].”

    From PCSK9 regulates neuronal apoptosis by adjusting ApoER2 levels and signaling (2012) · cites this paper as [25]

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Authors
Krämer, D., & Minichiello, L.
Affiliations
European Molecular Biology Laboratory (IT)
ORCID
Minichiello, Liliana
Venue
Methods in Molecular Biology
Publisher
Humana Press
Published
2010
Volume
633
Pages
233-239
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
DOI
10.1007/978-1-59745-019-5_17
OpenAlex
W2104721678
Cited by
51
References
0
Citations by year
2026: 3 · 2025: 4 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 7 · 2019: 1 · 2018: 6 · 2017: 3 · 2016: 6 · 2015: 3 · 2014: 2 · 2013: 4 · 2012: 1
FWCI
2.73
Type
book chapter
Language
en
Open access
closed
Locations
  • Methods in molecular biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Keywords
cerebellar granule cells, primary cell culture, neuronal development, neuronal apoptosis, neuroprotection, glutamate receptors, glutamate release, in vitro neuronal model
MeSH terms
Animals, Cell Separation, Cerebellum, Dissection, Neurons, Trypsin, Cell Culture Techniques, Mice, Rats

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