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Cited by 106CRISPR and Genetic EngineeringAnimal Genetics and ReproductionVirus-based gene therapy research

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Site-Specific Recombinases for Manipulation of the Mouse Genome

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Birling, Marie-Christine, Gofflot, Françoise, Warot, Xavier·2009·Methods in Molecular Biology
DOI 10.1007/978-1-60327-019-9_16
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Abstract

Site-specific recombination systems are widespread and popular tools for all scientists interested in manipulating the mouse genome. In this chapter, we focus on the use of site-specific recombinases (SSR) to unravel the function of genes of the mouse. In the first part, we review the most commonly used SSR, Cre and Flp, as well as the newly developed systems such as Dre and PhiC31, and we present the inducible SSR systems. As experience has shown that these systems are not as straightforward as expected, particular attention is paid to facts and artefacts associated with their production and applications to study the mouse genome. In the next part of this chapter, we illustrate new applications of SSRs that allow engineering of the mouse genome with more and more precision, including the FLEX and the RMCE strategies. We conclude and suggest a workflow procedure that can be followed when using SSR to create your mouse model of interest. Together, these strategies and procedures provide the basis for a wide variety of studies that will ultimately lead to the analysis of the function of a gene at the cellular level in the mouse.

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Metadata

Authors
Birling, M.-C., Gofflot, F., & Warot, X.
Affiliations
Phénotypage et ingénierie précliniques des systèmes d’organismes modèles (FR)
ORCID
Venue
Methods in Molecular Biology
Publisher
Humana Press
Published
2009
Volume
561
Pages
245-263
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
DOI
10.1007/978-1-60327-019-9_16
OpenAlex
W1747944491
Cited by
106
References
2
Citations by year
2026: 2 · 2025: 4 · 2024: 3 · 2023: 6 · 2022: 2 · 2021: 4 · 2020: 8 · 2019: 2 · 2018: 4 · 2017: 7 · 2016: 6 · 2015: 7 · 2014: 8 · 2013: 9 · 2012: 14
FWCI
13.46
Type
book chapter
Language
en
Open access
Licence
other-oa
Locations
  • Methods in molecular biology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • DIAL (Catholic University of Leuven) (submittedVersion)
  • Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Keywords
site-specific recombination, Cre recombinase, Flp recombinase, phiC31 integrase, conditional gene targeting, mouse model
MeSH terms
Animals, DNA Nucleotidyltransferases, Genetic Engineering, Mutagenesis, Genome, Gene Targeting, Animals, Genetically Modified, Mice

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Birling, M.-C., Gofflot, F., & Warot, X. (2009). Site-Specific Recombinases for Manipulation of the Mouse Genome. Methods in Molecular Biology. https://doi.org/10.1007/978-1-60327-019-9_16