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Reverse Genetics of Influenza Viruses
Abstract
The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility. Moreover, this technology is now being used to generate approved influenza virus vaccines and develop novel vaccines to combat seasonal and (future) pandemic influenza viruses. Several variations of the original system have been established, all of which are considerably robust and efficient.
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From Human innovation and the creative agency of the world in the age of generative AI (2024) · cites this paper as [10]
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Research works printed but not linked: 11
- Neumann G, Watanabe T, Ito H, Watanabe S, Goto H, Gao P, et al. Generation of influenza A viruses entirely from cloned cDNAs. Proc Natl Acad Sci USA. 1999 Aug 3;96(16):9345–50. · DOI
- Hoffmann E, Neumann G, Kawaoka Y, Hobom G, Webster RG. A DNA transfection system for generation of influenza A virus from eight plasmids. Proc Natl Acad Sci USA. 2000 May 23;97(11):6108–13. · DOI
- Neumann G, Fujii K, Kino Y, Kawaoka Y. An improved reverse genetics system for influenza A virus generation and its implications for vaccine production. Proc Natl Acad Sci USA. 2005 Nov 15;102(46):16825–9. · DOI
- Ozawa M, Goto H, Horimoto T, Kawaoka Y. An adenovirus vector-mediated reverse genetics system for influenza A virus generation. J Virol. 2007 Sep;81(17):9556–9. · DOI
- de Wit E, Spronken MI, Vervaet G, Rimmelzwaan GF, Osterhaus AD, Fouchier RA. A reverse-genetics system for Influenza A virus using T7 RNA polymerase. J Gen Virol. 2007 Apr;88(Pt 4):1281–7. · DOI
- Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR. Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol. 2001 Dec;146(12):2275–89. · DOI
- Niwa H, Yamamura K, Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 1991 Dec 15;108(2):193–9. · DOI
- Massin P, Rodrigues P, Marasescu M, van der Werf S, Naffakh N. Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza A viruses in avian cells. J Virol. 2005 Nov;79(21):13811–6. · DOI
- Murakami S, Horimoto T, Yamada S, Kakugawa S, Goto H, Kawaoka Y. Establishment of canine RNA polymerase I-driven reverse genetics for influenza A virus: its application for H5N1 vaccine production. J Virol. 2008 Feb;82(3):1605–9. · DOI
- Wang Z, Duke GM. Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells. Virol J. 2007;4:102. · DOI
- Neumann G, Watanabe T, Kawaoka Y. Plasmid-driven formation of influenza virus-like particles. J Virol. 2000 Jan;74(1):547–51. · DOI
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Human innovation and the creative agency of the world in the age of generative AI
Peschl, M. F. (2024) · Possibility Studies & Society · doi:10.1177/27538699241238049 · Cited by 30
Metadata
- Authors
- Neumann, G., Ozawa, M., & Kawaoka, Y.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Kawaoka, Yoshihiro
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2012
- Volume
- 865
- Pages
- 193-206
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W15834657
- Cited by
- 24
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 3 · 2015: 1 · 2013: 3
- FWCI
- 5.61
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (24658245)
- Topics
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- influenza virus, reverse genetics, reassortant viruses, mutant virus, viral replication, virulence, pathogenicity, host range, transmissibility, influenza vaccination, pandemic influenza
- MeSH terms
- Cloning, Molecular, Humans, Influenza, Human, Nucleoproteins, Orthomyxoviridae, Plasmids, DNA-Directed RNA Polymerases, Transfection, Viral Proteins, Virus Replication, Reassortant Viruses, DNA Primers, Reverse Transcriptase Polymerase Chain Reaction, Host Specificity, Reverse Genetics
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