Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture
Open accessAbstract
Retinal pigment epithelial (RPE) cells are among the most actively phagocytic cells in nature. Primary RPE and stable RPE cell lines provide experimental model systems that possess the same phagocytic machinery as RPE in situ. Upon experimental challenge with isolated photoreceptor outer segment fragments (POS), these cells promptly and efficiently recognize, bind, internalize, and digest POS. Here, we describe experimental procedures to isolate POS from porcine eyes and to feed POS to RPE cells in culture. Furthermore, we describe three different and complementary methods to quantify total POS uptake by RPE cells and to discriminate surface-bound from engulfed POS.
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The RPE-J cell line is a reliable cell culture system for studying RPE LAP and phagocytosis.
“we utilized a reliable cell culture system 13,28”
From RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye (2017) · cites this paper as [28]
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References 11 · Cited by 70
- Authors
- Mao, Y., & Finnemann, S. C.
- Affiliations
- Fordham University (US)
- Corresponding author
- Finnemann, Silvia C.
- Venue
- Methods in Molecular Biology
- Publisher
- Humana Press
- Published
- 2012
- Volume
- 935
- Pages
- 285-295
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- PMC
- PMC3590840
- OpenAlex
- W1593864683
- Cited by
- 70
- References
- 11
- Citations by year
- 2025: 4 · 2023: 5 · 2022: 8 · 2021: 4 · 2020: 5 · 2019: 8 · 2018: 17 · 2017: 11 · 2016: 1 · 2015: 1 · 2014: 6
- FWCI
- 10.37
- Type
- book chapter
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Eye Institute (R01EY013295, EY013295)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Keywords
- retinal pigment epithelium, photoreceptor outer segment phagocytosis, porcine eyes, retinal degeneration
- MeSH terms
- Animals, Cell Separation, Cells, Cultured, Microscopy, Fluorescence, Phagocytosis, Swine, Immunoblotting, Cell Culture Techniques, Retinal Pigment Epithelium, Retinal Photoreceptor Cell Outer Segment, Opsins
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