Diffuse Neuronal Perikaryon Amyloid Precursor Protein Immunoreactivity in a Focal Head Impact Model
Abstract
Amyloid precursor protein (APP) has been shown to accumulate in traumatically injured axons as early as 1 hour after injury. This accumulation may be due to interruption of fast axoplasmic transport and/or upregulation of APP synthesis. The aim of this study was to examine the neuronal cell body response to head impact using APP immunostaining in a focal non-missile head impact model. Ten anaesthetised and ventilated 2 year old Merino ewes were subjected to graded impact in the left temporal region by captive bolt. 2 hours after impact the brain was perfused fixed with formaldehyde. The tissue was mounted in paraffin, sectioned and stained with a monoclonal antibody to APP and standard H&E stain. APP positivity was semi-quantitated using a modification of our previously described sector scoring system [1]. Widespread neuronal APP positivity was found in the cerebral hemispheres and brain stem distant from the site of focal injury in all 10 animals. The most prominent APP positivity was found in the nerve cell bodies of the impacted left cerebral hemisphere. APP positive neurons were also found within regions which were structurally normal when stained with H&E. These results demonstrate diffuse neuronal perikaryon APP immunoreactivity following a focal head impact injury. The expression of APP within the neuronal cell body may be due to upregulation of APP synthesis or alterations in the availability of epitopes of APP. Further studies are in progress to address these hypothe…
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APP expression increases in striatal and hippocampal axons along with cortical and thalamic neurons within the first 24 h after experimental impact and fluid percussion TBI.
“For example, APP expression increases in striatal and hippocampal axons along with cortical and thalamic neurons within the first 24 h after experimental impact and fluid percussion TBI (121–123)”
From The Inflammatory Continuum of Traumatic Brain Injury and Alzheimer’s Disease (2018) · cites this paper as [123]
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References 4 · Cited by 25
- Authors
- Heuvel, C., Lewis, S., Wong, M., Manavis, J., Finnie, J., Blumbergs, P., Jones, N., & Reilly, P.
- Affiliations
- South Australia Pathology (AU)
- Royal Adelaide Hospital (AU)
- Venue
- Intracranial Pressure and Neuromonitoring in Brain Injury
- Publisher
- Springer Vienna
- Published
- 1998
- Volume
- 71
- Pages
- 209-211
- OpenAlex
- W2113824597
- Cited by
- 25
- References
- 4
- Citations by year
- 2026: 1 · 2023: 1 · 2021: 1 · 2019: 1 · 2018: 3 · 2016: 1 · 2014: 1
- FWCI
- 3.70
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Springer eBooks (publishedVersion)
- PubMed (publishedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- Topics
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Keywords
- amyloid precursor protein, head impact injury, traumatic brain injury, ewes, cerebral hemispheres, brainstem, axonal transport
- MeSH terms
- Animals, Axons, Brain, Female, Immunoenzyme Techniques, Neurons, Sheep, Temporal Lobe, Head Injuries, Closed, Amyloid beta-Protein Precursor
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