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Cited by 20Barrier Structure and Function StudiesAluminum toxicity and tolerance in plants and animalsHeavy Metal Exposure and Toxicity

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

Cytochemical study of the effect of aluminium on cultured brain microvascular endothelial cells

Vorbrodt, Andrzej W., Trowbridge, Richard S., Dobrogowska, Danuta H.·1994·The Histochemical Journal
DOI 10.1007/bf00157960
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Abstract

The cytotoxic effect of aluminium was studied on cultured goat brain microvascular endothelial cells used as an in vitro model of the blood-brain barrier. Confluent monolayers of these cells were exposed for 4 days to aluminium maltol and, for control purposes, to maltol alone, and also to cadmium chloride as a known cytotoxic substance. The localization of plasmalemma-bound enzymatic activities of 5'-nucleotidase and Ca(2+)-ATPase and the distribution of sialic acid residues were studied at the ultrastructural level. It was observed that the reaction for 5'-nucleotidase activity was only insignificantly affected, indicating its resistance to the cytotoxic action of both substances used. On the contrary, the activity of Ca(2+)-ATPase was evidently suppressed, especially in the interendothelial clefts where junctional complexes are presumably to be formed. Aluminium also affects the density of sialic acid residues, as shown by their redistribution, leading to the appearance of relatively long segments of unlabelled apical cell surface. The data obtained suggest that observed changes in the localization of Ca(2+)-ATPase and sialic acid residues can lead ultimately to impairment of the formation and maintenance of intercellular junctions and to disturbances in the negatively charged domains of the endothelial cell surface. Whether these alterations, induced in vitro, contribute to in vivo disturbances of blood-brain barrier function requires further experimental study.

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Crossref · 10.1007/bf00157960

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  1. Kang, Y. H. & Watson, L. P. (1988) Ultracytochemical study of the effect of bacterial endotoxin and calmodulin on Ca2+-ATPase activity in human NK cells. J. Histochem. Cytochem. 36, 908.

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Metadata

Authors
Vorbrodt, A. W., Trowbridge, R. S., & Dobrogowska, D. H.
Affiliations
New York State Office for People With Developmental Disabilities (US)
Venue
The Histochemical Journal
Publisher
Springer Science and Business Media LLC
Published
1994-02
Volume
26
Issue
2
Pages
119-126
ISSN
  • 0018-2214 (ISSN-L)
  • 1573-6865
DOI
10.1007/bf00157960
OpenAlex
W1966514857
Cited by
20
References
24
Citations by year
2019: 1 · 2018: 1 · 2015: 1
FWCI
0.73
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • The Histochemical Journal (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Aluminum toxicity and tolerance in plants and animals
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
Keywords
aluminum toxicity, brain microvascular endothelial cells, blood-brain barrier, cytotoxicity, Ca2+-ATPase, 5'-nucleotidase, sialic acid, interendothelial clefts, intercellular junctions, endothelial ultrastructure, cadmium chloride
MeSH terms
Calcium-Transporting ATPases, Aluminum, Animals, Blood-Brain Barrier, Brain, Cadmium, Cell Membrane, Cells, Cultured, Chlorides, Endothelium, Vascular, Goats, Immunohistochemistry, Microscopy, Electron, Organometallic Compounds, Pyrones, Sialic Acids, 5'-Nucleotidase, N-Acetylneuraminic Acid, Cadmium Chloride

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