The ATP- and tolbutamide-sensitivity of the ATP-sensitive K-channel from human pancreatic B cells
Open accessAbstract
The ATP- and sulphonylurea-sensitivity of the ATP-sensitive K-channel was measured in human pancreatic B cells. In inside-out patches, half-maximal inhibition of channel activity was produced by 10 mumol/l ATP (with 2 mM Mg2+) and ATP-inhibition was partially antagonised by ADP. A significantly lower sensitivity to ATP was found in whole-cell recordings. Tolbutamide inhibited whole-cell ATP-sensitive K-currents half-maximally at 18 mumol/l; the sensitivity to tolbutamide was somewhat less in the inside-out patch. Ca-activated K-channels were unaffected by tolbutamide (10 mmol/l). These results resemble those found for rodent B cells and suggest that sulphonylureas exert their therapeutic effects in Type 2 (non-insulin dependent) diabetes by inhibition of the ATP-sensitive K-channel.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The whole-cell KATP conductance in the absence of glucose in human β-cells is <10% of that found in mouse β-cells, being around 60 pS/pF, and this difference is likely due to a lower open probability.
“However, the whole-cell KATP conductance in the absence of glucose is <10% of that found in mouse β-cells, being around 60 pS/pF (535). As there is no major difference in single-channel conductance or channel density (28), the difference is likely to be due to a lower open probability.”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [28]
The Discussion (page unavailable) reports very low initial whole-cell conductance in intact human β-cells, supporting the general point that few KATP channels are active. But it gives less than 50 pS per cell, not around 60 pS/pF; using the 7.6 pF mean capacitance reported in Whole-cell currents (page unavailable) yields less than about 7 pS/pF. The paper does not establish the claimed <10% comparison with mouse β-cells. The lower-open-probability explanation follows a separate citation in the citing sentence, so it is not assessed as an attribution to this paper. (LLM, conf=0.84)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cited by
Sorted by citations
Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
Self-citation
- Authors
- Ashcroft, F. M., Kakei, M., Gibson, J. S., Gray, D. W., & Sutton, R.
- Affiliations
- John Radcliffe Hospital (GB)
- ORCID
- Ashcroft, F.M.
- Venue
- Diabetologia
- Published
- 1989-08
- Volume
- 32
- Issue
- 8
- Pages
- 591-8
- ISSN
- 0012-186X (ISSN-L)
- 1432-0428
- OpenAlex
- W2116167295
- Cited by
- 39
- References
- 0
- Citations by year
- 2026: 1 · 2023: 1 · 2018: 3 · 2017: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 1.68
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Diabetologia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Keywords
- ATP-sensitive potassium channels, sulfonylurea receptor, tolbutamide, ATP inhibition, calcium-activated potassium channels, type 2 diabetes, insulin secretion
- MeSH terms
- Adenosine Triphosphate, Calcium, Humans, Islets of Langerhans, Kinetics, Membrane Potentials, Tolbutamide, Potassium Channels, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
