The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells
Abstract
When metaphase PtK1 cells are cooled to 6-8 degrees C for 4-6 h the free, polar, and astral spindle microtubules (MTs) disassemble while the MTs of each kinetochore fiber cluster together and persist as bundles of cold-stable MTs. These cold-stable kinetochore fibers are similar to untreated kinetochore fibers in both their length (i.e., 5-6 micrometer) and in the number of kinetochore-associated MTs (i.e., 20-45) of which they are comprised. Quantitative information concerning the lengths of MTs within these fibers was obtained by tracking individual MTs between serial transverse sections. Approximately 1/2 of the kinetochore MTs in each fiber were found to run uninterrupted into the polar region of the spindle. It can be inferred from this and other data that a substantial number of MTs run uninterrupted between the kinetochore and the polar region in untreated metaphase PtK1 cells.
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A cold treatment assay confirmed the localization of StableMARK to kinetochore fibers.
“A cold treatment assay (Rieder 1981; Ohta et al., 2015) confirmed the localization of StableMARK to kinetochore fibers (Fig. S6 A).”
From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Rieder 1981
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A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle
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- Authors
- Rieder, C. L.
- Affiliations
- New York State Department of Health (US)
- Corresponding author
- Rieder, Conly L.
- Venue
- Chromosoma
- Published
- 1981-09
- Volume
- 84
- Issue
- 1
- Pages
- 145-158
- ISSN
- 0009-5915 (ISSN-L)
- 1432-0886
- OpenAlex
- W2068843702
- Cited by
- 228
- References
- 0
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 8 · 2021: 12 · 2020: 8 · 2019: 8 · 2018: 8 · 2017: 8 · 2016: 12 · 2015: 6 · 2014: 7 · 2013: 13 · 2012: 7
- FWCI
- 2.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Chromosoma (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- kinetochore fibers, metaphase spindle, PtK1 cells, microtubule disassembly, microtubule length, astral microtubules, microtubule tracking
- MeSH terms
- Animals, Cell Line, Cold Temperature, Kidney, Marsupialia, Metaphase, Microscopy, Electron, Microtubules
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