A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for tRNA nonsense suppressors
Abstract
A spontaneous mutant was isolated that harbors a weak suppressing activity towards a UAG mutation, together with an inability to grow at 43 degrees C in rich medium. The mutation is shown to be associated with an increased misreading of UAG at certain codon contexts and UAA. UGA, missense or frameshift mutations do not appear to be misread to a similar extent. The mutation gives an increased efficiency to several amber tRNA suppressors without increasing their ambiguity towards UAA. The ochre suppressors SuB and Su5 are stimulated in their reading of both UAG and UAA with preference for UAG. An opal suppressor is not affected. The effect of the mutation on the efficiency of amber and ochre suppressors is dependent on the codon context of the nonsense codon. The mutated gene (uar) has been mapped and found to be recessive both with respect to suppressor-enhancing ability as well as for temperature sensitivity. The phenotype is partly suppressed by the ochre suppressor SuC. It is suggested that uar codes for a protein, which is involved in translational termination at UAG and UAA stop codons.
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The readthrough of UAA occurs at frequencies from 9 × 10−4 to less than 1 × 10−5.
“The readthrough of UAA occurs at frequencies from 9 × 10−4 to less than 1 × 10−5 [70].”
From Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines (2021) · cites this paper as [70]
Natural UAG readthrough frequency is mostly within the range of 1.1 × 10−4 to 7 × 10−3, depending on the nature of the downstream nucleotides.
“Natural UAG readthrough frequency is mostly within the range of 1.1 × 10−4 to 7 × 10−3, depending on the nature of the downstream nucleotides [70,71,72,73].”
From Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines (2021) · cites this paper as [70]
Readthrough also occurs with stop codons UAG and UAA in bacteria, but at a lower rate than UGA.
“Readthrough also occurs with stop codons UAG and UAA in bacteria [69,70], but at a lower rate.”
From Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines (2021) · cites this paper as [70]
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- Authors
- Rydén, S. M., & Isaksson, L. A.
- Affiliations
- Uppsala University (SE)
- Venue
- Molecular and General Genetics MGG
- Published
- 1984-01
- Volume
- 193
- Issue
- 1
- Pages
- 38-45
- ISSN
- 0026-8925 (ISSN-L)
- 1432-1874
- OpenAlex
- W2049135896
- Cited by
- 129
- References
- 0
- Citations by year
- 2026: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 10 · 2017: 3 · 2016: 2 · 2015: 6 · 2014: 5 · 2013: 2 · 2012: 8
- FWCI
- 0.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular and General Genetics MGG (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
- Keywords
- nonsense codon suppression, amber suppressor tRNA, temperature-sensitive mutants, translation termination, codon context, Escherichia coli
- MeSH terms
- beta-Glucosidase, Chromosomes, Bacterial, Codon, Crosses, Genetic, Escherichia coli, Genes, Bacterial, Mutation, RNA, Messenger, RNA, Transfer, Species Specificity, Suppression, Genetic, Temperature, Transduction, Genetic
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