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Cited by 15Pancreatic function and diabetesCellular transport and secretionIon channel regulation and function

Coupling of exocytosis to depolarization in rat pancreatic islet ?-cells: effects of Ca2+, Sr2+ and Ba2+-containing extracellular solutions

Barnett, David W., Misler, Stanley·1995·Pfl�gers Archiv European Journal of Physiology
DOI 10.1007/bf00373898
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Abstract

Using rat beta-cells we present evidence that Sr2+ and Ba2+, like Ca2+, support depolarization-induced increases in membrane capacitance which reflect insulin granule exocytosis. Even with identical total charge entry, Sr2+ and Ba2+ are 3-5 and 20-fold less effective than Ca2+ in supporting release. While exocytosis supported by Sr2+ is graded with cation entry and complete within 250ms of depolarization, exocytosis supported by Ba2+ begins abruptly after a threshold of charge entry and continues for many seconds. Ba(2+)-supported release continues in the presence of greatly enhanced cytosolic Ca2+ buffering, arguing against release of Ca2+ from stores as its principal action. These results suggest that Sr2+ and Ba2+ support exocytosis largely by binding to Ca(2+)-dependent release-activating sites, though with less affinity than Ca2+.

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Crossref · 10.1007/bf00373898

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  • The elevation of [Ca2+]i resulting from opening of Ca2+ channels leads to activation of BK channels and initiates action potential repolarization.

    “The elevation of [Ca2+]i resulting from the opening of the Ca2+ channels leads to activation of BK channels and initiates action potential repolarization (51, 90).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [51]

  • At the peak of the action potential (-20 mV and above), P/Q-type Ca2+ channels open and trigger exocytosis of insulin granules.

    “At the peak of the action potential (-20 mV and above), P/Q-type Ca2+ channels open and trigger exocytosis of insulin granules (51, 90).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [51]

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Authors
Barnett, D. W., & Misler, S.
Affiliations
Washington University in St. Louis (US)
ORCID
Barnett, David W.
Venue
Pfl�gers Archiv European Journal of Physiology
Publisher
Springer Science and Business Media LLC
Published
1995-08
Volume
430
Issue
4
Pages
593-595
ISSN
  • 0031-6768 (ISSN-L)
  • 1432-2013
DOI
10.1007/bf00373898
OpenAlex
W2045439447
Cited by
15
References
0
Citations by year
2017: 1
FWCI
2.13
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Pflügers Archiv - European Journal of Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01dk037380-12)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-37380)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
insulin exocytosis, pancreatic beta cells, depolarization, membrane capacitance, calcium, strontium, barium, intracellular calcium stores
MeSH terms
Animals, Barium, Calcium, Cations, Divalent, Cell Membrane, Culture Media, Cytosol, Exocytosis, Extracellular Space, Islets of Langerhans, Strontium, Patch-Clamp Techniques, Rats, In Vitro Techniques

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