An electrophoretic study of the distribution and properties of human hexokinases
Abstract
An electrophoretic system which gives a clear separation of human hexokinases HK1, HK2 and HK3 is described. The distribution of the hexokinase isozymes in various human tissues, both adult and fetal, is reported. Some properties of the isozymes were investigated. HK2 was found to be more thermolabile than HK1, and there was also a small but significant difference in molecular size. Unlike HK3, HK1 and HK2 are not inhibited by high glucose concentrations. Screening of red cell lysates from 800 unrelated European individuals revealed no genetic variants of HK1 and HK2. However, in view of their difference in properties, it seems probable that the HK1 and HK2 isozymes are determined by separate gene loci.
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Hexokinase isoenzymes HK-I-IV catalyze the first committed step of glucose metabolism, converting free glucose to glucose-6-phosphate.
“Hexokinase isoenzymes (HK-I–IV) catalyze the first committed step of glucose metabolism, the intracellular conversion of free glucose to glucose-6-phosphate (Glu-6-P) [183, 184, 186, 198].”
From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [186]
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References 20 · Cited by 44
- Authors
- Rogers, P. A., Fisher, R. A., & Harris, H.
- Affiliations
- University College London (GB)
- ORCID
- Harris, Harry
- Venue
- Biochemical Genetics
- Published
- 1975-12
- Volume
- 13
- Issue
- 11-12
- Pages
- 857-866
- ISSN
- 0006-2928 (ISSN-L)
- 1573-4927
- OpenAlex
- W2083735549
- Cited by
- 44
- References
- 20
- Citations by year
- 2024: 1 · 2018: 3 · 2015: 3 · 2013: 1
- FWCI
- 5.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biochemical Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Glycogen Storage Diseases and Myoclonus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- hexokinase, hexokinase isozymes, hexokinase 1, HK2, HK3, isozyme separation, tissue distribution, fetal tissue, thermolability, molecular size, glucose inhibition, genetic variants, genetic loci, erythrocytes, human
- MeSH terms
- Brain, Drug Stability, Electrophoresis, Starch Gel, Erythrocytes, Female, Fetus, Hot Temperature, Hexokinase, Humans, Isoenzymes, Leukocytes, Liver, Male, Molecular Weight, Muscles, Myocardium, Organ Specificity, Pregnancy
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