Heme-biosynthetic enzyme activities and porphyrin accumulation in normal liver and hepatoma cell lines of rat
Open accessAbstract
The activities of four heme-biosynthetic enzymes, delta-aminolevulinic acid (ALA) synthase, ALA dehydratase, porphobilogen (PBG) deaminase, and ferrochelatase, were studied in five epithelial cell lines of normal rat liver origin (RL, RLC-10, RLC-24, M, Culb-TC) and five cell lines derived from Yoshida ascites hepatoma (JTC-1, JTC-2, JTC-15, JTC-16, JTC-24). The JTC series of hepatoma-derived cell lines exhibited decreased ALA synthase activity and increased ALA dehydratase activity, although the activities of all four enzymes and the Km values for their respective substrates varied widely from one cell line to another, a finding suggesting that specific regulatory mechanisms for porphyrin metabolism might operate in each cell type. M cells, which were transformed by 4-dimethylaminoazobenzene in vitro, gave the most abnormal Km values of heme-biosynthetic enzymes among all the cell lines studies, and were found to accumulate hematoporphyrin derivative (HpD).
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Enhanced activity of enzymes leading to production of PpIX, such as ALAD, UROD, or PBGD, has been observed in tumor cells.
“Enhanced activity of enzymes leading to production of PpIX, such as ALAD [43], UROD [43], or PBGD [38,43,44] has also been observed in tumor cells.”
From Aminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer (2011) · cites this paper as [44]
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References 8 · Cited by 69
- Authors
- Kondo, M., Hirota, N., Takaoka, T., & Kajiwara, M.
- Affiliations
- Tokyo Metropolitan Institute of Public Health (JP)
- Jichi Medical University (JP)
- Meiji (Japan) (JP)
- Venue
- Cell Biology and Toxicology
- Published
- 1993
- Volume
- 9
- Issue
- 1
- Pages
- 95-105
- ISSN
- 0742-2091 (ISSN-L)
- 1573-6822
- OpenAlex
- W2059871838
- Cited by
- 69
- References
- 8
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 1 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-nd
- www.springer.com/tdm (tdm)
- Locations
- Cell Biology and Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Funders
- Japan Science and Technology Agency
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
- Physical Sciences › Materials Science › Materials Chemistry › Porphyrin and Phthalocyanine Chemistry
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Keywords
- heme biosynthesis, porphyrin metabolism, delta-aminolevulinic acid synthase, delta-aminolevulinic acid dehydratase, porphobilinogen deaminase, ferrochelatase, hepatoma cell lines, rat liver, enzyme kinetics, substrate Km, 4-dimethylaminoazobenzene, M cells, Yoshida ascites hepatoma
- MeSH terms
- Porphobilinogen Synthase, 5-Aminolevulinate Synthetase, Animals, Cell Line, Ferrochelatase, Heme, Carcinoma, Hepatocellular, Kinetics, Liver, Hydroxymethylbilane Synthase, Porphyrins, Tumor Cells, Cultured, Rats
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