The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Sequence determination of the 3’ end of mouse mammary tumor virus RNA
Abstract
70S RNA has been prepared from mouse mammary tumor virus (MMTV) produced by the GR tumor cell line. After denaturation for 3 min at 60 degrees C the RNA was applied to a sucrose gradient and molecules sedimenting between 10-16S were selected and passed over an oligo-dT cellulose column. The poly A containing RNA fraction was used as a template for the synthesis of complementary DNA with reverse transcriptase in the presence of dideoxynucleoside triphosphates. Oligo-nucleotides p(dT)7rG, p(dT)7rA and (p(dT)7rC were tested for their primer activity. Two of the three primers gave readable sequences. This suggests a heterogeneity in the 3' end of the viral RNA, a phenomenon also observed with avian retroviruses. Nucleotides 37-45 from the 3' end are made up of only A's and T's and resemble a Hogness box. This finding could have biological consequences for the transcription of proviral DNA in the unintegrated and integrated state. Instead of the usual AAUAAA sequence present in mRNA's close and in front of the site of polyadenylation we find a sequence AGUAAA.
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“It is worth mentioning that in the case of MMTV, instead of the conserved hexamer AAUAAA, an alternative AGUAAA hexamer is located ∼20 nts upstream of the transcriptional start site, serving as the polyA signal (Klemenz et al. 1981, Coffin 1997).”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Klemenz et al. 1981
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“It is worth mentioning that in the case of MMTV, instead of the conserved hexamer AAUAAA, an alternative AGUAAA hexamer is located ∼ 20 nts upstream of the transcriptional start site, serving as the polyA signal (Klemenz et al. 1981 , Coffin 1997 ).”
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Metadata
- Authors
- Klemenz, R., Reinhardt, M., & Diggelmann, H.
- Venue
- Molecular Biology Reports
- Published
- 1981
- Volume
- 7
- Issue
- 1-3
- Pages
- 123-126
- ISSN
- 0301-4851 (ISSN-L)
- 1573-4978
- OpenAlex
- W2060232992
- Cited by
- 24
- References
- 19
- Citations by year
- 2025: 1 · 2013: 1
- FWCI
- 1.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular Biology Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Keywords
- mouse mammary tumor virus, RNA sequence determination, polyadenylation signal, RNA heterogeneity, retroviral RNA
- MeSH terms
- Animals, Base Sequence, Cell Line, Centrifugation, Density Gradient, DNA, Female, Hot Temperature, Mammary Tumor Virus, Mouse, Mammary Neoplasms, Experimental, Oligodeoxyribonucleotides, Poly A, RNA, RNA, Messenger, RNA, Viral, Mice
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